Evidence map›Paper›PMID 41187058›Full record

ArticleCell reports2025

Radial astroglia cooperate with microglia to clear neuronal cell bodies during zebrafish optic tectum development.

Heather M Barber, Cassidy G Robbins, Zachary Cutler, Robin I Brown, Lauren E Jung, Inge Werkman, Sarah Kucenas

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Heather M BarberCell & Developmental Biology Graduate Program, University of Virginia, Charlottesville, VA 22903, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Cassidy G RobbinsDepartment of Biology, University of Virginia, Charlottesville, VA 22904, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Zachary CutlerDepartment of Biology, University of Virginia, Charlottesville, VA 22904, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Robin I BrownDepartment of Biology, University of Virginia, Charlottesville, VA 22904, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Lauren E JungCell & Developmental Biology Graduate Program, University of Virginia, Charlottesville, VA 22903, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Inge WerkmanDepartment of Biology, University of Virginia, Charlottesville, VA 22904, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA.
Sarah KucenasDepartment of Biology, University of Virginia, Charlottesville, VA 22904, USA; Cell & Developmental Biology Graduate Program, University of Virginia, Charlottesville, VA 22903, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA 22904, USA; University of Virginia Brain Institute, Charlottesville, VA 22904, USA. Electronic address: sk4ub@virginia.edu.

Funding

Characterization of the cellular and molecular mechanisms that mediate glial tilingR01NS107525 · NINDS · UNIVERSITY OF VIRGINIA · PI KUCENAS, SARAH C · 2019 to 2023
$1.7M
Radial Astroglia Form Novel Structures to Engulf Neuronal Cell Bodies during Zebrafish Optic Tectum DevelopmentF31EY035961 · NEI · UNIVERSITY OF VIRGINIA · PI BARBER, HEATHER M · 2024 to 2025
$83k
NEI NIH HHS F31 EY035961NINDS NIH HHS R01 NS107525
6 · The paper itself

Abstract

The clearance of dead cells by phagocytes is an essential component of neural development in many organisms. Microglia are the main phagocytes in the central nervous system (CNS), but the extent of participation by other glial cells remains unclear, especially under homeostatic conditions. During zebrafish optic tectum (OT) development, we observed radial astroglia forming dynamic, spherical projections from their basal processes. These projections, which we call scyllate heads, coincide with a wave of neuronal cell death in the OT. We show that scyllate heads surround the majority of dying neurons soon after phosphatidylserine exposure. However, unlike traditional phagosomes, scyllate heads persist for many hours and are rarely acidified or internalized. Instead, microglia invade scyllate heads and remove their contents for degradation. Our study reveals an active role for radial astroglia in homeostatic cell clearance and cooperation between microglia and radial astroglia during zebrafish OT development.

Indexed as

AstrocytesCell BodyMicrogliaNeuronsSuperior ColliculiZebrafishAnimalsPhagocytosisastrocyteastrogliaCP: Developmental biologyCP: Neurosciencegliamicroglianeural developmentoptic tectumphagocytosisprogrammed cell deathradial gliazebrafish

Identifiers

PMID41187058
PMCPMC12752020

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.