Evidence map›Paper›PMID 41186628›Full record

ArticleCell and tissue research2025

Downregulation of ankyrin 3 (ANK3) promotes malignant behaviors associated with altered adhesion dynamics and actin cytoskeleton remodeling in renal cell carcinoma.

Keerakarn Somsuan, Artitaya Rongjumnong, Wararat Chiangjong, Atthapan Morchang, Arunothai Wanta, Ratirath Samol, Sirirat Yongrum, Natthiya Sakulsak, Anupong Makeudom, Suttichai Krisanaprakornkit and 4 more

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Article in Cell and tissue research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

14 authors.

Keerakarn SomsuanSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Artitaya RongjumnongSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Wararat ChiangjongPediatric Translational Research Unit, Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
Atthapan MorchangSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Arunothai WantaSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Ratirath SamolDepartment of Anatomical Pathology, Sawanpracharak Hospital, Nakornsawan, 60000, Thailand.
Sirirat YongrumSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Natthiya SakulsakFaculty of Medicine, Praboromarajchanok Institute, Nonthaburi, 11000, Thailand.
Anupong MakeudomSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Suttichai KrisanaprakornkitSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Sutatip PongcharoenDepartment of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, 65000, Thailand.
Somchai ChutipongtanateDepartment of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH, 45267-0056, USA.
Jatuporn NgoenkamDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand. jatupornn@nu.ac.th.
Siripat AluksanasuwanSchool of Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand. siripat.alu@mfu.ac.th.

Funding

National Research Council of Thailand N42A660849
6 · The paper itself

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer, with existing therapies largely ineffective for advanced stages. Ankyrin 3 (ANK3), a protein involved in various cellular processes, has been associated with poor prognosis and immune infiltration in ccRCC. However, its role in ccRCC development and progression remains poorly understood. In the present study, ANK3-knockdown 786-O cells were generated using a shRNA approach. Cellular assays revealed that ANK3 knockdown significantly enhanced cell proliferation, migration, and invasion compared to shControl cells. ANK3-knockdown cells also displayed increased nuclear size and accumulation of G2/M phase of the cell cycle. Proteomic analysis identified 59 significantly altered proteins in ANK3-knockdown cells, primarily involved in cell adhesion and structural integrity. Among these, 10 proteins were significantly associated with overall survival in ccRCC patients. Morphological analysis showed that ANK3-knockdown cells exhibited a more spindle-like shape and increased cellular protrusions (filopodia). Western blot analysis further demonstrated altered expression levels of junctional and cytoskeleton proteins, including decreased E-cadherin and increased ZO-1, ICAM-1, vimentin, and β-actin. Immunofluorescence analysis revealed the accumulation of F-actin in the cytoplasm and filopodia in ANK3-knockdown cells. Additionally, ANK3 knockdown enhanced cell adhesion and aggregation capabilities. Finally, a decrease in ANK3 expression was confirmed in ccRCC tissues (grades I and II) compared to adjacent normal tissues. These findings suggest that ANK3 acts as a potential modulator of cell adhesion and cytoskeleton remodeling in ccRCC and may represent a promising therapeutic target for this malignancy.

Indexed as

Actin CytoskeletonAnkyrinsCarcinoma, Renal CellDown-RegulationKidney NeoplasmsCell AdhesionCell Line, TumorCell MovementCell ProliferationHumansMaleANK3 protein, humanAnkyrinsAnkyrin 3Cell adhesionProteomicsRenal cell carcinomaTumor suppressor

Identifiers

PMID41186628

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.