Evidence map›Paper›PMID 41184795›Full record

ArticleBMC plant biology2025

Transcription factor MdWRKY50 correlates with spur-type formation in apple by modulating endogenous gibberellin biosynthesis.

Tianci Yan, Kun Shi, Ruoxue Li, Yixue Bai, Xiaodong Zheng, Dongqian Shan, Awei Jiang, Chanyu Wang, Haixia Zhang, Zehui Hu and 4 more

Abstract read
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In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

14 authors.

Tianci YanCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Kun ShiCollege of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia, 010021, China.
Ruoxue LiCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Yixue BaiCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Xiaodong ZhengCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Dongqian ShanCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Awei JiangCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Chanyu WangCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Haixia ZhangCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Zehui HuCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Yanzhao SunCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China.
Xiangshu PiaoCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Yan GuoCollege of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Jin KongCollege of Horticulture, China Agricultural University, No.2 Yuan Ming Yuan Xi Road, Beijing, 100193, China. jinkong@cau.edu.cn.

Funding

Beijing Science and Technology Program Z231100003723002Hainan Province Science and Technology Special Fund ZDYF2021XDNY161National Key Research & Development Program of China 2022YFF1003100National Natural Science Fund 32172540Sanya Ya-zhou Bay Science and Technology City SYND-2021-08the 2115 Talent Development Program of China Agricultural University, and the 111 Project B17043
6 · The paper itself

Abstract

The spur-type trait is an important breeding goal for apple (Malus × domestica Borkh.) cultivar, due to its advantages in yield and orchard mechanical management. MdWRKY50 encoding a transcription factor were identified to have dramatically lower expression in spur type. RNA interference of it leads to significantly shortened internodes in transgenic apple plants, while its overexpression resulted in opposite phenotype. The shortened internode of transgenic apple with RNA interfered MdWRKY50 was in good correlation with the bioactive gibberellin (GA) level. Furthermore, MdWRKY50 was proved to directly binds to the promoter of MdGA3ox, encoding a rate-limiting biosynthetase in GA biosynthesis, as confirmed by Chromatin immunoprecipitation followed by quantitative PCR (ChIP-qPCR) and Electrophoretic Mobility Shift Assay (EMSA). This binding up-regulated the expression of MdGA3ox. The low expression of MdWRKY50-MdGA3ox regulatory pathway is conserved across six spur-type apple cultivars in contrast to the high expressions across six standard-type apple cultivars. Our findings established a MdWRKY50-MdGA3ox module regulating GA-mediated internode elongation and coorelating with spur-type formation, which might contribute to the molecular breeding of spur-type cultivars adapted to high-density, mechanized apple orchards.

Indexed as

GibberellinsMalusPlant ProteinsTranscription FactorsGene Expression Regulation, PlantPlants, Genetically ModifiedGibberellinsPlant ProteinsTranscription FactorsAppleGibberellin biosynthesisMdGA3oxMdWRKY50Spur-type

Identifiers

PMID41184795
PMCPMC12581608

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.