Evidence map›Paper›PMID 41184588›Full record

ArticleThe EMBO journal2025

Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.

Yashna Thappeta, Silvia J Cañas-Duarte, Haozhen Wang, Till Kallem, Alessio Fragasso, Yingjie Xiang, William Gray, Cheyenne Lee, Georgeos Hardo, Lynette Cegelski and 1 more

Abstract read
In one paragraph

Article in The EMBO journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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  13. Coupling of cell growth modulation to asymmetric division and cell cycle regulation inProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Yashna Thappeta *Sarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
Silvia J Cañas-Duarte *Sarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-5705-0023
Haozhen WangSarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
Till KallemDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Alessio FragassoSarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
Yingjie XiangMechanical Engineering and Materials Science, Yale University, New Haven, CT, USA.
William GrayDepartment of Pharmacology, Yale University, New Haven, CT, USA.
Cheyenne LeeMicrobial Sciences Institute, Yale University, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-6891-3266
Georgeos HardoDepartment of Engineering, University of Cambridge, Cambridge, Cambridgeshire, UK.ORCID http://orcid.org/0000-0003-0037-1293
Lynette CegelskiDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Christine Jacobs-WagnerSarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA. jacobs-wagner@stanford.edu.ORCID http://orcid.org/0000-0003-0980-5334

Funding

Bacterial Cell Wall Composition and the Influence of AntibioticsR01GM117278 · NIGMS · STANFORD UNIVERSITY · PI CEGELSKI, LYNETTE S · 2016 to 2023
$2.6M
HHS | National Institutes of Health (NIH) R01GM117278Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) 019.222EN.001NIGMS NIH HHS R01 GM117278UKRI | Biotechnology and Biological Sciences Research Council (BBSRC) BB/M011194/1University of Cambridge (cambridgeuniversity) Strategic Seed Fund
6 · The paper itself

Abstract

Bacteria often experience nutrient limitation. While the exponential and stationary growth phases have been characterized in the model bacterium Escherichia coli, little is known about what happens inside individual cells during the transition between these two phases. Through quantitative cell imaging, we found that the positions of nucleoids and cell division sites become increasingly asymmetric during the transition phase. These asymmetries were accompanied by an asymmetric reorganization of protein, ribosome, and RNA probes in the cytoplasm. Results from live-cell imaging experiments, complemented with genetic and

Indexed as

Asymmetric Cell DivisionCell DivisionEscherichia coliGlycogenCytoplasmEscherichia coli ProteinsMagnetic Resonance SpectroscopyMicroscopy, Atomic ForceEscherichia coli ProteinsGlycogenAsymmetric DivisionBacteriaGlycogenNutrient LimitationPhase Separation

Identifiers

PMID41184588
PMCPMC12706056

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.