Evidence map›Paper›PMID 41183564›Full record

ArticleStem cells translational medicine2025

Anti-fibrotic effect of human amniotic fluid stem cells in biliary epithelial-mesenchymal transition of liver ductal organoid.

Sinobol Chusilp, Poramate Klanrit, Carol Lee, Dorothy Lee, Bo Li, Felicia Balsamo, Kanokrat Thaiwatcharamas, Patchareeporn Tanming, Dolrudee Aroonsaeng, Paisarn Vejchapipat and 1 more

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Article in Stem cells translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sinobol ChusilpDivision of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.ORCID 0000-0001-6473-0385
Poramate KlanritDepartment of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Carol LeeDivision of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
Dorothy LeeDivision of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
Bo LiDivision of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.ORCID 0000-0002-8298-8344
Felicia BalsamoDivision of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
Kanokrat ThaiwatcharamasDivision of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Patchareeporn TanmingDivision of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Dolrudee AroonsaengDivision of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Paisarn VejchapipatDivision of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Agostino PierroDivision of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIn biliary atresia (BA), it has been demonstrated that biliary epithelial-mesenchymal transition (EMT) of reactive ductular cells is associated with liver fibrosis. This study aimed to develop an ex vivo biliary EMT model of liver ductal organoids for exploring the involvement of biliary EMT in fibrogenesis and to investigate whether human amniotic fluid stem cells (hAFSCs) can mitigate the biliary EMT process.

methodsLiver ductal organoids were generated from the intrahepatic bile duct of healthy neonatal mice. Biliary EMT was induced in organoids by the administration of transforming growth factor beta-1 (TGF-β1) in culture medium. hAFSCs were co-cultured with organoids during biliary EMT induction. Expression of biliary epithelial cells, mesenchymal cells, myofibroblast, collagen I, and genes related to the Wnt signaling pathway were evaluated.

resultsFollowing administration of TGF-β1, we observed an increased expression of mesenchymal cell markers N-cadherin and Vimentin, as well as myofibroblast marker alpha-smooth muscle actin (α-SMA) in liver ductal organoids which were associated with increased expression of collagen 1. Administration of hAFSCs to organoids significantly attenuated TGF-β1-induced biliary EMT and collagen production. In addition, Wnt signaling was upregulated in biliary EMT, while hAFSCs downregulated the Wnt signaling resulting in decreased expression of myofibroblast and collagen in organoids.

conclusionTGF-β1 is a potent cytokine that induces biliary EMT. hAFSCs significantly mitigated TGF-β1-induced biliary EMT in liver ductal organoids. The beneficial effect of hAFSCs administration is associated with the downregulation of the Wnt signaling pathway. This study indicates that hAFSCs can prevent the progression of liver fibrosis in BA.

Indexed as

Amniotic FluidEpithelial-Mesenchymal TransitionLiverLiver CirrhosisOrganoidsStem CellsAnimalsHumansMiceTransforming Growth Factor beta1Wnt Signaling PathwayTransforming Growth Factor beta1amniotic fluid stem cellbile ductepithelial-mesenchymal transitionorganoids

Identifiers

PMID41183564
PMCPMC12582591

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.