ArticleMethods in molecular biology (Clifton, N.J.)2026
Identifying Trypanosoma cruzi Proteins Targeted by Chagas Disease Patient Antibodies Using Yeast Surface Display.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Understanding and identifying protein-ligand interactions is essential for elucidating fundamental biological processes and developing translational applications such as vaccine and drug targets. Yeast surface display (YSD) systems expressing genome-wide or combinatorial libraries in Saccharomyces cerevisiae are powerful tools to identify protein-ligand interactions in an unbiased fashion. In the following protocol, we couple the YSD system expressing a genome-wide library of the protozoan pathogen Trypanosoma cruzi with magnetic-activated cell sorting to identify antigen-antibody interactions. We describe the enrichment of pathogen antigens targeted by antibodies of infected patients using the YSD library. We also detailed a DNA sequencing methodology and a data analysis computational pipeline. The approach can be easily adapted to identify protein-protein or protein-drug interactions.
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