ArticleMethods in molecular biology (Clifton, N.J.)2026
Identification of Trypanosoma cruzi Discrete Typing Units by Multiplex Real-Time PCR.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma cruzi isolates are classified into six discrete typing units (DTUs), TcI to TcVI, and TcBat. An accurate identification of T. cruzi genotype is critical for understanding its epidemiology, pathogenesis, and response to treatment. Several typing methodologies have been developed with variable levels of complexity, selectivity, and analytical sensitivity. This chapter describes a standardized multiplex real-time polymerase chain reaction (PCR) protocol using TaqMan probes to identify parasite DTUs based on the analysis of T. cruzi SL-IR, 18S rDNA, COII, and 24Sα rDNA targets. This methodology can identify the six main DTUs directly from clinical and biological specimens in a single or two sequential qPCR reactions with high sensitivity and specificity, minimizing contamination risks and reducing the number of steps required. It offers a simplified approach to genotyping that is adaptable for field studies and clinical diagnostics.
Indexed as
Identifiers
41182614What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.