ArticleApplied biochemistry and biotechnology2025
Mannobiose Production Through Synergistic Enzymatic Hydrolysis of Locust Bean Gum by Mannanases from Alkaliphilic Bacillus sp. N16-5.
Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Recent Advances in the Manufacturing of Furfurylamine Through Biological and Chemobiological Ways.Applied biochemistry and biotechnology · 2026Review
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10 authors.
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Abstract
β-(1,4)-Mannobiose is a functional oligosaccharide with multiple prebiotic properties. The limitation of existing production technologies has hindered its further development and applications. In this study, we developed an efficient and scalable method combining a three-step enzymatic hydrolysis process with microbial purification to produce high-purity mannobiose from locust bean gum (LBG). The enzymatic degradation was carried out using β-mannanases rManA and rMan113A, along with α-galactosidase rGal27A from the alkaliphilic Bacillus N16-5, sequentially breaking down the substrate into galactose, mannose, and mannobiose. Lactobacillus casei LH23 was then employed to selectively metabolize mannose and galactose, ensuring the exclusive production of mannobiose. In a 250 mL reaction system containing 5% LBG, mannobiose was obtained at a concentration of 25.99 g/L with a conversion efficiency of 51.98%, representing 98% of the total sugar yield. This study highlights the significance of understanding microbial polysaccharide utilization and engineering specialized enzymes to enhance enzymatic hydrolysis strategies for targeted oligosaccharide production.
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