Evidence map›Paper›PMID 41181323›Full record

ArticleFrontiers in cellular and infection microbiology2025

LpxR and PagL expression in live attenuated auxotrophic

Miriam Moscoso, Víctor Fuentes-Valverde, Patricia García, Juan A Vallejo, Jesús Pérez-Ortega, Rebeca Santamarina-Fernández, Ana Candela, Marina Oviaño, Jesús Arenas, Germán Bou

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Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Engineering low-endotoxin lipid A in a double auxotrophFrontiers in cellular and infection microbiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Miriam Moscoso *Servicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Víctor Fuentes-Valverde *Servicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Patricia GarcíaServicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Juan A VallejoServicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Jesús Pérez-OrtegaDepartment of Molecular Microbiology, Faculty of Science and Institute of Biomembranes. Utrecht University, Utrecht, Netherlands.
Rebeca Santamarina-FernándezServicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Ana CandelaServicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Marina OviañoServicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.
Jesús Arenas *Department of Molecular Microbiology, Faculty of Science and Institute of Biomembranes. Utrecht University, Utrecht, Netherlands.
Germán Bou *Servicio de Microbiología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, A Coruña, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: Two lipid A-modifying enzymes, LpxR and PagL were overproduced in PAO1 Δ Results: LPS extracted from the wild-type strain showed heterogeneous lipid A structures with varying degrees of acylation, and a predominant penta-acylated species. Expression of LpxR led to enrichment in tetra-acylated species, while PagL expression reduced the heterogeneity observed in the wild type. Both mutant strains showed decreased TLR4 activation Discussion: Lipid A modifications mediated by LpxR and PagL attenuated TLR4 signaling

Indexed as

AcyltransferasesBacterial ProteinsImmunogenicity, VaccineLipid APseudomonas aeruginosaPseudomonas InfectionsPseudomonas VaccinesAnimalsDisease Models, AnimalFemaleHEK293 CellsHumansLipopolysaccharidesMiceToll-Like Receptor 4Vaccines, AttenuatedAcyltransferasesBacterial ProteinsLipid ALipopolysaccharidesPseudomonas VaccinesToll-Like Receptor 4Vaccines, AttenuatedD-glutamate auxotrophyhumoral immunitylipid A modifying-enzymeslipopolysaccharidelive vaccinesprotective efficacyPseudomonas aeruginosareactogenicity

Identifiers

PMID41181323
PMCPMC12571757

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.