Evidence map›Paper›PMID 41179998›Full record

ArticleDevice2025

Scalable Bioelectronic Production of Extracellular Vesicles for Cardiac Tissue Regeneration.

Lingyuan Meng, Jing Zhang, Pengju Li, Wen Li, Jiuyun Shi, Zhe Cheng, Eleanor Ostroff, Chuanwang Yang, Jiping Yue, Aleksander Prominski and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Device, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lingyuan MengPritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, USA.
Jing ZhangThe James Franck Institute, University of Chicago, Chicago, IL 60637, USA.
Pengju LiPritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, USA.
Wen LiDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Jiuyun ShiDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Zhe ChengDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Eleanor OstroffDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Chuanwang YangThe James Franck Institute, University of Chicago, Chicago, IL 60637, USA.
Jiping YueDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Aleksander ProminskiDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Saehyun KimDepartment of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Jiwang ChenCardiovascular Research Center, University of Illinois at Chicago, Chicago, IL 60637, USA.
Bozhi TianThe James Franck Institute, University of Chicago, Chicago, IL 60637, USA.

Funding

Multi-site photostimulation devices using porosity-based semiconductor heterojunctions for cardiac resynchronization therapyR01EB036091 · NIBIB · UNIVERSITY OF CHICAGO · PI Narutoshi Hibino, Bozhi Tian · 2024 to 2026
$1.8M
Updating Echocardiograph Imaging Instrument at University of Illinois ChicagoS10OD034370 · OD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI JIN, JIAN-PING · 2024 to 2024
$516k
NIBIB NIH HHS R01 EB036091NIH HHS S10 OD034370
6 · The paper itself

Abstract

EV-based therapies are hindered by low production efficiency and poor scalability. Conventional methods to enhance EV yield-such as hypoxia or chemical stimulation-often compromise vesicle quality and cell health. This study introduces a bioelectronic platform featuring a planar interdigitated electrode array that enables simultaneous low-voltage (±1 V), low-frequency (2 Hz) biphasic stimulation and real-time super-resolution imaging of EV biogenesis via TIRF microscopy. This bioelectrical stimulation on primary cardiac cells significantly increases EV secretion without affecting cell viability. The resulting electrically-induced EVs (e-EVs) exhibit enhanced microRNA cargo-loading capacity, preserved tissue tropism, and functional therapeutic potential. In a murine model of acute myocardial infarction, miRNA-loaded e-EVs improved cardiac function and reduced fibrosis. These results highlight the potential of bioelectronic modulation as a scalable, non- destructive strategy for improving EV yield and functional performance in translational regenerative medicine.

Identifiers

PMID41179998
PMCPMC12574593

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.