Evidence map›Paper›PMID 41179315›Full record

ArticleCancer diagnosis & prognosis

Podoplanin - Related Lymphatic Micro Vessel Density Digital Analysis in Breast Adenocarcinoma.

Georgios I Metaxas, Spyridon Marinopoulos, Maria Adamopoulou, Kalliroi Goula, Ioannis K Papapanagiotou, Evangelos Tsiambas, Sofianiki Mastronikoli, Eftychia Papachatzopoulou, Panagiotis Fotiades, Athanasios Niotis and 3 more

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Article in Cancer diagnosis & prognosis. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Georgios I Metaxas *First Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.
Spyridon Marinopoulos *First Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.
Maria AdamopoulouDepartment of Science and Mathematics, Biomedical Sciences, Deree-The American College of Greece, Athens, Greece.
Kalliroi Goula *Department of Pathology, ''Alexandra'' General Hospital of Athens, Athens, Greece.
Ioannis K PapapanagiotouFirst Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.
Evangelos Tsiambas *Department of Cytology, 417 Veterans Army Hospital (NIMTS), Athens, Greece.
Sofianiki Mastronikoli *Brighton and Sussex Medical School, Brighton, U.K.
Eftychia PapachatzopoulouFirst Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.
Panagiotis Fotiades *Department of Surgery, 424 General Army Hospital, Thessaloniki, Greece.
Athanasios Niotis1st Department of Pathology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
George TsouvelasDepartment of Psychology, National and Kapodistrian University of Athens, Athens, Greece.
Sofia KouraFirst Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.
Constantine DimitrakakisFirst Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, "Alexandra" General Hospital, Athens, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background/Aim: Neo-lymphangiogenesis induces lymphatic invasion of cancer cells, significantly increasing the metastatic potential of breast carcinomas (BCs). Among the molecules that are implicated in lymphangiogenesis, podoplanin (PDPN, gene locus: 1p36.21) - a transmembrane receptor glycoprotein - is expressed exclusively in lymphatic vessels. The current study explored the impact of PDPN-dependent mean lymphatic micro-vessel density (mLMVD) in invasive ductal (inDBC) and invasive lobular breast adenocarcinomas (inLBC). Materials and Methods: A set of thirty (n=30) paraffin-embedded invasive BC tissue sections (22 inDBCs and 8 inLBCs, respectively) were analyzed by applying a combination of immunocytochemistry (IHC) and digital image analysis (DIA) assays. Results: High and moderate mLMVD rates (defined by the mean number of lymphatic domains with emboli in five optical fields under 400X magnification) were detected in 5/30 (16.6%) and 6/30 (20%) cases (total 11/30 (36.6%), respectively. In these cases, PDPN demonstrated strong cytoplasmic/membranous staining intensity. The remaining 19 cases (63.4%) demonstrated low levels of mLMVD. mLMVD was significantly correlated with the stage of the examined malignancies ( Conclusion: Neo-lymphangiogenesis in BCs is a critical histological feature in the progression of the malignancy and is correlated with an aggressive phenotype (advanced stage). PDPN expression in lymphatic micro vessels is a reliable biomarker for evaluating lymphangiogenic activity in BCs, independently of their histotype, especially when assessed with precise DIA techniques.

Indexed as

biomarkersbreastcarcinomadigital analysisimmunohistochemistrylymphangiogenesisPodoplanin

Identifiers

PMID41179315
PMCPMC12577617

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