Evidence map›Paper›PMID 41178446›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Depletion of Sorcs3 Activates Totipotency in Mouse Embryonic Stem Cells by Modulating Key Signaling Pathways.

Wenhao Zhang, Xinyu Mao, Yu He, Qingshen Jia, Yiding Zhao, Xiaomeng Dai, Xiaoyan Li, Shengyi Sun, Xiaoyan Sheng, Dan Ding and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wenhao ZhangState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Xinyu MaoState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Yu HeDepartment of Neonatology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Chongqing, 400014, China.
Qingshen JiaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Yiding ZhaoState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Xiaomeng DaiState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Xiaoyan LiState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Shengyi SunState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Xiaoyan ShengState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Dan DingState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Yuan ShiDepartment of Neonatology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Chongqing, 400014, China.
Qian GaoState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Ling ShuaiState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Animal Resources Center and Reproductive Regulation, and Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.ORCID https://orcid.org/0000-0003-0671-7478

Funding

Fundamental Research Funds for the Central Universities 63223030Institute of Transplantation Medicine NKTM2023003Key Project of Tianjin Science and Technology 24ZXZSSS00150National Key Research and Development Program of China 2023YFC2705900National Natural Science Foundation of China 82001602National Natural Science Foundation of China 82201840National Natural Science Foundation of China 82301879National Natural Science Foundation of China 82371671Natural Science Foundation of Chongqing CSTB2024NSCQ-MSX0491Shanghai Key Laboratory of Reproduction and Development Redlab202401
6 · The paper itself

Abstract

Totipotency represents the greatest potential to yield an entire individual alongside its associated extraembryonic tissues, albeit transiently. Nevertheless, achieving sustainable totipotent stem cells remains an intriguing yet challenging endeavor. Here, it is reported that Sorcs3 depletion in murine embryonic stem cells (ESCs) enables robust differentiation into both embryonic and extraembryonic lineages, resulting in a totipotent-like state. Notably, Sorcs3 knockout (SKO)-ESCs can efficiently self-assemble into typical blastocyst-like structures, offering a versatile model for studying early embryonic development. Comprehensive analyses reveal that totipotency in SKO-ESCs is related to Tfap2c gene activation. Deletion of Tfap2c significantly reduces the developmental potential of SKO-ESCs across all the examined phenotypes, underscoring its critical role. Furthermore, single-cell transcriptome analysis of SKO-ESCs reveals that inhibition of the TGF-β, PI3K-AKT, and lysosome pathways drives totipotency activation, which is validated by the introduction of corresponding inhibitors into wild-type ESC cultures. Together, the findings facilitate the establishment of totipotent stem cells in a defined medium and provide a universal platform for studying totipotency.

Indexed as

Mouse Embryonic Stem CellsSignal TransductionAnimalsCell DifferentiationEmbryonic DevelopmentMiceTranscription Factor AP-2Transcription Factor AP-2ESCssignaling pathwaySorcs3Tfap2ctotipotency

Identifiers

PMID41178446
PMCPMC12866853

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.