ArticlePhysiological reports2025
Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice.
Article in Physiological reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- The Impact of Overtraining on Anxiety-Like Behavior and Tight Junction Proteins of the Blood-Brain Barrier in Mice.Brain and behavior · 2026Article
- Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice.Physiological reports · 2025Article
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Authors and funding
8 authors.
Funding
Abstract
Overtraining, which results in central nervous system (CNS)-fatigue-related symptoms, leads to a long-term decline in performance. This study investigated CNS-derived EV protein content under overtraining conditions to identify potential biomarkers. Eight-week-old C57BL/6J mice were randomly divided into three groups: sedentary (SED, n = 7), exercise control (EX, n = 8), and overtraining (OT, n = 9) groups. The OT group underwent an 8-week downhill treadmill-based overtraining induction protocol. Exercise capacity was assessed using the incremental load, exhaustion, grip strength, and rotarod tests, while motor deficits, depression and anxiety were assessed using the nest building test. Proinflammatory cytokines were measured in blood plasma, skeletal muscle, and brain tissue. CNS-derived EVs were isolated using a two-step EV isolation protocol. Isolated EVs underwent proteomic analyses. Mice exhibited a significant decrease in aerobic exercise capacity, high-intensity exercise tolerance, and muscular strength. OT increased quadriceps IL-6 and hypothalamic IL-1β/TNFα compared to EX. Plasma IL-2 levels tended to be higher in OT than in EX. Proteomic analysis of CNS-derived EVs revealed a decrease in lipid metabolism-related proteins and an increase in stress-related proteins. Valosin-containing proteins and catalase, which are upregulated in organs under oxidative stress, were increased. Therefore, CNS-derived EV protein contents indicated CNS fatigue under overtraining conditions.
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