Evidence map›Paper›PMID 41177849›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2026

Discovery of IS1515 transposition into the Spn9802 fragment of Streptococcus pneumoniae and its implications for PCR-based diagnostic assays.

Myrthe M A Toorop, Margriet E M Kraakman, Eric C J Claas, Els Wessels, Stefan A Boers

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Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Myrthe M A TooropLeiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands.
Margriet E M KraakmanLeiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands.
Eric C J ClaasLeiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands.
Els WesselsLeiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands.
Stefan A BoersLeiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands. s.a.boers@lumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigates a diagnostic challenge in detecting Streptococcus pneumoniae using a Spn9802-targeted PCR assay. Four S. pneumoniae isolates tested lytA-positive but Spn9802-negative due to an insertion sequence (IS1515) within Spn9802, which was confirmed by whole-genome sequencing. This insertion may lead to false-negative results, posing concerns for PCR-based diagnostics. The study emphasizes the need for dual-target PCR and regular updates to diagnostic primers, underscoring the importance of continuous assay evaluation and post-market surveillance to ensure reliable pathogen detection.

Indexed as

DNA Transposable ElementsPneumococcal InfectionsPolymerase Chain ReactionStreptococcus pneumoniaeDNA, BacterialHumansMolecular Diagnostic TechniquesWhole Genome SequencingDNA, BacterialDNA Transposable Elements

Identifiers

PMID41177849

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.