Evidence map›Paper›PMID 41177834›Full record

ArticleNeurogastroenterology and motility2025

Altered Sigmoid Mucosal Innervation and Mast Cell Proximity to Sensory Nerve Fibers Are Associated With Symptom Severity in Patients With Irritable Bowel Syndrome.

Pu-Qing Yuan, Michael Nash, Tao Li, Swapna Mahurkar-Joshi, Jessica Sohn, Ariela Khandadash, Yvette Taché, Lin Chang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurogastroenterology and motility, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pu-Qing YuanDigestive Diseases Research Center, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Michael NashG. Oppenheimer Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Tao LiDigestive Diseases Research Center, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Swapna Mahurkar-JoshiG. Oppenheimer Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Jessica SohnG. Oppenheimer Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Ariela KhandadashG. Oppenheimer Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Yvette TachéDigestive Diseases Research Center, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Lin ChangG. Oppenheimer Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0001-6800-2967

Funding

Comprehensive Structural and Functional Mapping of Mammalian Colonic Nervous SystemOT2OD024899 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MULUGETA, MILLION, TACHE, YVETTE FRANCE · 2017 to 2021
$13.1M
NIH HHS OT2 OD024899NIH SPARC OD024899
6 · The paper itself

Abstract

backgroundPeripheral neuronal pathways contribute to the pathophysiology of irritable bowel syndrome (IBS).

aimsTo use high-resolution three-dimensional (3D) imaging and computerized quantification to compare the density and proximity of distinct nerve fibers (NFs) to mast cells (MCs) in sigmoid mucosal biopsies from patients with constipation-predominant IBS (IBS-C), diarrhea-predominant IBS (IBS-D), and healthy controls (HCs).

methodsSigmoid biopsies from 23 IBS patients (10 IBS-C [6 females]; 13 IBS-D [7 females]) and 12 HCs (6 females) were processed, NFs and MCs were immunostained and 3D images were analyzed using Imaris software to quantify NF density and proximity to MCs. Data were correlated with the IBS Severity Scoring System (IBS-SSS) and abdominal pain ratings.

resultsCompared to HCs, IBS patients had reduced densities of human peripheral choline acetyltransferase (hpChAT, p = 0.01) and calbindin (Calb, p = 0.08) NFs by 39% and 17%, respectively, with the most prominent reductions of 50% (p = 0.004) and 26% (p = 0.03) in IBS-C. Vasoactive intestinal peptide (VIP) NF density was significantly higher in IBS-D compared to IBS-C (p = 0.01). Other NF densities and MC count did not significantly differ between IBS and HCs. IBS-SSS and abdominal pain scores were positively correlated with the proximity of MCs to SP (p = 0.038) and Calb (p = 0.047) NFs and negatively with the density of VIP (p = 0.039) and NPY (p = 0.005) NFs. CONCLUSIONS & INFERENCES: In IBS, there are reduced densities of colonic enteric cholinergic and intrinsic primary afferent NFs. Correlations between abdominal pain severity and MC-NF interactions in IBS patients suggest that altered neuroimmune signaling may contribute to IBS pathophysiology and represent a potential therapeutic target.

Indexed as

Colon, SigmoidIntestinal MucosaIrritable Bowel SyndromeMast CellsNerve FibersSensory Receptor CellsAdultFemaleHumansMaleMiddle AgedSeverity of Illness IndexYoung Adultabdominal painenteric cholinergic fibersirritable bowel syndromemast cellssubstance Pvasoactive intestinal peptide

Identifiers

PMID41177834
PMCPMC12623276

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.