Evidence map›Paper›PMID 41176226›Full record

ArticleJournal of advanced research2026

The effector AGLIP1 hijacks peroxidase OsAPX8 to coordinate rice immune suppression and ROS-Driven cell death for facilitating Rhizoctonia solani infection.

Zhuangyuan Fu, Junyi Ma, Bing Cao, Jun Yang, Shubin Li, Jun Wang, Wenxin Jiang, Yufeng Jia, Lekang Dong, Wei Cheng and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zhuangyuan FuSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Junyi MaSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Bing CaoSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Jun YangInstitute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shubin LiDepartment of Plant Pathology, Shenyang Agricultural University, Liaoning 110866, China.
Jun WangDepartment of Plant Pathology, Shenyang Agricultural University, Liaoning 110866, China.
Wenxin JiangSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Yufeng JiaSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Lekang DongSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Wei ChengSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China.
Jiyang WangDepartment of Plant Pathology, State Key Laboratory of Agricultural and Forestry Biosecurity, China Agricultural University, Beijing 100193, China.
Shuai LiSanya National Center of Technology Innovation for Saline-Alkali Tolerant Rice, Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Hainan 572025, China. Electronic address: aqlishuai@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionNecrotrophic fungi such as Rhizoctonia solani, the causal agent of rice sheath blight (RSB), release effectors that suppress host immune defenses and trigger host cell death. However, the molecular basis for this dual functionality remains poorly understood.

objectivesThis study aimed to characterize the effector AGLIP1 from R. solani, elucidate its dual role in modulating rice immune responses and cell death, and investigate the molecular mechanism by which it promotes infection.

methodsWe combined transgenic over-expression lines, CRISPR-Cas9 knockouts, host-induced gene-silenced (HIGS) plants, pathogenesis assays, interaction proteomics, enzyme-kinetics measurements, and confocal imaging to dissect AGLIP1 activity and its impact on OsAPX8.

resultsWe showed the effector AGLIP1 from R. solani, and reveal that it simultaneously dampens basal immune responses and triggers reactive oxygen species (ROS)-mediated cell death in rice. Functional assays demonstrated that AGLIP1 expression in rice cells suppresses defense-associated gene expression and leads to excessive ROS accumulation. Consistently, HIGS of AGLIP1 significantly enhanced rice resistance to sheath blight, confirming that AGLIP1 is a crucial virulence factor. Furthermore, AGLIP1 physically interacts with the rice ascorbate peroxidase OsAPX8, a chloroplast-targeted enzyme which positively regulates RSB resistance. AGLIP1 binding inhibits the peroxidase activity of OsAPX8 and relocalizes this enzyme from chloroplasts to peroxisomes, compromising the host's ROS-scavenging defenses. Moreover, overexpression of OsAPX8 in plant cells attenuates AGLIP1-induced cell death, supporting the notion that AGLIP1 promotes cell death by inactivating and misdirecting OsAPX8.

conclusionOur findings uncover a novel virulence mechanism utilized by R. solani, where the effector AGLIP1 subverts host antioxidant defenses via OsAPX8 inhibition and mislocalization, facilitating pathogen infection. This insight highlights potential breeding targets, suggesting strategies to stabilize OsAPX8 function or prevent its subversion by AGLIP1 for developing rice cultivars with improved resistance to sheath blight.

Indexed as

Fungal ProteinsOryzaPlant DiseasesPlant ProteinsReactive Oxygen SpeciesRhizoctoniaCell DeathHost-Pathogen InteractionsPlant ImmunityPlants, Genetically ModifiedFungal ProteinsPlant ProteinsReactive Oxygen SpeciesAscorbate peroxidaseEffectorNecrotrophic pathogensReactive oxygen speciesRice sheath blight

Identifiers

PMID41176226
PMCPMC13316532

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.