Evidence map›Paper›PMID 41175307›Full record

ArticleJournal of biomolecular NMR2025

Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in

Yoshiki Shigemitsu, Yuki Miyazaki, Hibiki Terami, Daria Ostapova, Tatsuya Matsunaga, Ryo Takahashi, Takumi Inoue, Toshio Yamazaki, Yoshitaka Ishii

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yoshiki Shigemitsu *School of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Yuki Miyazaki *School of Life Science and Technology, Tokyo Institute of Technology, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Hibiki Terami *School of Life Science and Technology, Tokyo Institute of Technology, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Daria OstapovaSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Tatsuya MatsunagaSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Ryo TakahashiSchool of Life Science and Technology, Tokyo Institute of Technology, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Takumi InoueSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan.
Toshio YamazakiBDR Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Kanagawa, Japan.
Yoshitaka IshiiSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Midori-ku, Yokohama, 226-8503, Kanagawa, Japan. ishii@life.isct.ac.jp.ORCID http://orcid.org/0000-0002-7724-6469

Funding

Japan Society for the Promotion of Science JP15K21772, JapanJST-Mirai Program JPMJMI17A2, Japan
6 · The paper itself

Abstract

We present a novel approach for side-chain-selective deuteration of proteins to improve

Indexed as

DeuteriumNuclear Magnetic Resonance, BiomolecularProteinsEscherichia coliIsotope LabelingDeuteriumProteinsIsotope labelingMedium-switchingSelective protonationSolid-state NMR

Identifiers

PMID41175307
PMCPMC12664835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.