Evidence map›Paper›PMID 41175240›Full record

ArticleApplied microbiology and biotechnology2025

Virus-like particle-based liquid phase blocking ELISA for evaluating the efficacy of O-type foot-and-mouth disease vaccines.

Yun Zhang, Tian Wei, Mei Ren, Suyu Mu, Manyuan Bai, Shuanghui Yin, Shiqi Sun, Huichen Guo, Hu Dong

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yun Zhang *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Tian Wei *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Mei RenState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Suyu MuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Manyuan BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shuanghui YinState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shiqi SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Huichen GuoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Hu DongState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. donghu@caas.cn.

Funding

The Major Science and Technology Project of Gansu Province 24ZDWA004the Science and Technology Support Program of Gansu Province KJZC-2025-10Zhejiang Hom-Sun Biosciences Co., Ltd. Open Research Project ZJHS202510
6 · The paper itself

Abstract

Virus-like particles (VLPs), which closely mimic the structural and antigenic properties of native foot-and-mouth disease (FMD) virions while lacking infectious genetic material, provide a more economical solution by replacing inactivated viruses in liquid-phase blocking ELISA (LPBE) testing for FMD serodiagnosis and vaccine evaluation. In this study, a novel LPBE was developed by replacing inactivated antigens with serotype O foot-and-mouth disease virus (FMDV) VLPs expressed in Escherichia coli. Based on evaluation of sensitivity and specificity, its correlation with virus neutralization test (VNT) results in immunized animal sera, as well as the feasibility and broad applicability for clinical serum sample detection, was further analyzed. Validation showed that VLPs-LPBE achieved high specificity (98.75%) and sensitivity (97.02%). Notably, in quantifying VLP-induced antibodies, the correlation with VNT results (R

Indexed as

Foot-and-Mouth DiseaseFoot-and-Mouth Disease VirusVaccines, Virus-Like ParticleViral VaccinesAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayNeutralization TestsSensitivity and SpecificityAntibodies, ViralVaccines, Virus-Like ParticleViral VaccinesAntibody detectionFMDVLiquid-phase blocking ELISAVirus-like particles

Identifiers

PMID41175240
PMCPMC12579672

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.