Evidence map›Paper›PMID 41175225›Full record

ArticleArchives of microbiology2025

Evaluation of the vaccine formulation with recombinant protein CP01850 and fish oil from Rhamdia quelen: an alternative and innovative adjuvant against Corynebacterium pseudotuberculosis.

Nicole Ramos Scholl, Tallyson Barbosa Nogueira, Mirna Samara Dié Alves, Henrique Gonçalves Pegoraro, Fernanda Severo Sabedra Sousa, Fabiana Kommling Seixas, Tiago Collares, Danielle Tapia Bueno, Cláudio Martin de PereiraPereira, Sibele Borsuk

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nicole Ramos SchollLaboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário S/N, Prédio 19, Pelotas, RS, 96010-900, Brazil.
Tallyson Barbosa NogueiraLaboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário S/N, Prédio 19, Pelotas, RS, 96010-900, Brazil.
Mirna Samara Dié AlvesLaboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário S/N, Prédio 19, Pelotas, RS, 96010-900, Brazil.
Henrique Gonçalves PegoraroLaboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário S/N, Prédio 19, Pelotas, RS, 96010-900, Brazil.
Fernanda Severo Sabedra SousaLaboratório de Biotecnologia do Câncer, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS, 96010-900, Brazil.
Fabiana Kommling SeixasLaboratório de Biotecnologia do Câncer, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS, 96010-900, Brazil.
Tiago CollaresLaboratório de Biotecnologia do Câncer, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS, 96010-900, Brazil.
Danielle Tapia BuenoLaboratório de Lipidômica e Bio-Orgânica, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, UFPel, Pelotas, RS, 96010-900, Brazil.
Cláudio Martin de PereiraPereiraLaboratório de Lipidômica e Bio-Orgânica, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, UFPel, Pelotas, RS, 96010-900, Brazil.
Sibele BorsukLaboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário S/N, Prédio 19, Pelotas, RS, 96010-900, Brazil. sibeleborsuk@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fish oils contain bioactive molecules such as omega-3, -6, and - 9 fatty acids, which modulate immune responses. The oil of Rhamdia quelen (RQO) was characterized, revealing 62.86% unsaturated fatty acids. To assess its potential as an adjuvant in caseous lymphadenitis (CLA) vaccines, Balb/c mice were divided into four groups: saline (G1), rCP01850 + AddaVax (G2), rCP01850 + RQO (G3), and RQO alone (G4). Only G3 achieved significant protection (4/10, p = 0.0047), while G1 and G4 showed none. Humoral analysis showed that G3 had the second highest antibody production (total IgG, IgG2b, IgG3), surpassed only by G2. At day 42, IgG1 and IgG2a levels were statistically equivalent between G2 and G3. Importantly, G3 was the only group with elevated IFN-γ and IL-17 (p = 0.0001), indicating a robust cellular immune response. These findings suggest that RQO is a safe and promising natural adjuvant capable of enhancing both humoral and cellular responses, improving vaccine efficacy against CLA.

Indexed as

Adjuvants, ImmunologicAdjuvants, VaccineBacterial ProteinsBacterial VaccinesCorynebacterium InfectionsCorynebacterium pseudotuberculosisFish OilsAnimalsAntibodies, BacterialFemaleImmunoglobulin GInterferon-gammaMiceMice, Inbred BALB CRecombinant ProteinsAdjuvants, ImmunologicAdjuvants, VaccineAntibodies, BacterialBacterial ProteinsBacterial VaccinesFish OilsImmunoglobulin GInterferon-gammaRecombinant ProteinsAdjuvantsAntibodiesCaseous lymphadenitisFish oilImmunoprotectionSubunit vaccine

Identifiers

PMID41175225

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.