ArticleAnalytical and bioanalytical chemistry2025
Comparative evaluation of comprehensive offline 2D-LC strategies coupled to MS for untargeted metabolomic studies of human urine.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Capillary Electrophoresis and Atomic Absorption Spectrometry-A Rare Yet Valuable Liaison, Also from a Pharmaceutical Perspective.Molecules (Basel, Switzerland) · 2026Review
- Decoding the Metabolic Signatures of Neurodegeneration Diseases: Advances in Mass Spectrometry-Based Metabolomics.Metabolites · 2026Review
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
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Authors and funding
4 authors.
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Abstract
Out of the broad selection of analytical methods applied in metabolomic studies, liquid chromatography coupled to mass spectrometry (LC-MS) has the highest coverage potential. In that regard, the quality of the separation process is crucial for the analytical outcome. Reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) are widely applied, and the potential of various setups to combine these modes for more complementary data has been deeply explored. In our previous study on the orthogonality of LC conditions in the field of metabolomics, combinations of a mixed-mode phase with parallel RP and ion-exchange (IEX) properties and several HILIC columns exhibited the widest compound distributions in a two-dimensional (2D) separation space. For further performance evaluation, an offline comprehensive 2D-LC-TOF-MS (LC×LC-TOF-MS) system was set up with the mixed RP/IEX mode in the first dimension (
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