ArticleBMC oral health2025
Effect of melatonin on the osteogenic differentiation of periodontal ligament stem cells: in vitro study.
Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Melatonin-Hydrogel Delivery Systems for Effective Treatments of Periodontitis: Translational Perspectives from Material Designs to Clinical Applications.Gels (Basel, Switzerland) · 2026Review
- Histological evaluation of pulpal healing following direct pulp capping with polytetrafluoroethylene or melatonin, with or without adjunctive low-level laser therapy in Wistar rat molars.Scientific reports · 2026Article
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Authors and funding
3 authors.
Funding
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Abstract
backgroundPeriodontal regeneration is a viable treatment, but outcomes remain variable. Incorporating stem cells and bioactive agents could enhance regeneration. Human periodontal ligament stem cells (hPDLSCs) display osteogenic potential, and melatonin, a neurohormone, modulates osteoblast differentiation and stimulates osteogenesis in dental stem cells. However, the influence of melatonin dosage on hPDLSC differentiation is poorly understood.This study aims to evaluate the effects of varying melatonin concentrations on periodontal hPDLSC osteogenic differentiation.
methodshPDLSCs were cultured with melatonin at 0, 0.01, 10, 20, 30, 40, 50, 100, and 150 µM; viability (AlamarBlue) and differentiation (immunocytochemistry, ELISA, Alizarin Red) were assessed at 14 and 21 days.
resultsAll melatonin‑treated groups exhibited enhanced hPDLSC viability compared to the control. Cell viability decreased dose‑dependently, with the lowest viability in the 100 µM and 150 µM groups. Notably, 20 µM and 50 µM treatments deviated from this trend, showing significant viability increases. Collagen type I expression and calcium mineralization were observed in all groups, increasing over time, with maximal deposits at 21 days versus 14 days. The 20 µM and 50 µM groups demonstrated a statistically significant rise in mineral deposition after 21 days in osteogenic media.
conclusionMelatonin is biocompatible and dose-dependently stimulates hPDLSC viability. Melatonin concentrations of 20 and 50 µM were most effective for osteogenic differentiation of hPDLSCs. Our results show that melatonin directly accelerated the differentiation of human periodontal stem cells into osteoblasts and might be used as a pharmaceutical to promote bone regeneration.
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