Evidence map›Paper›PMID 41174663›Full record

ArticleBMC biology2025

Serotonin modulation of metabolism and stress response in Pseudomonas fluorescens.

Barbora Waclawiková, Markus Schwalbe, Diana Ilyaskina, Semih Toptas, Nicola U Thome, Chao Du, Somayah S Elsayed, Anne de Jong, Gilles P van Wezel, Sahar El Aidy

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In one paragraph

Article in BMC biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Barbora Waclawiková *Host-Microbe Metabolic Interactions, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.
Markus Schwalbe *Host-Microbe Metabolic Interactions, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.
Diana IlyaskinaDepartment of Microbiome Engineering, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Semih ToptasHost-Microbe Metabolic Interactions, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.
Nicola U ThomeDepartment of Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, The Netherlands.
Chao DuDepartment of Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, The Netherlands.
Somayah S ElsayedDepartment of Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, The Netherlands.
Anne de JongMolecular Genetics Group, University of Groningen, Groningen, The Netherlands.
Gilles P van WezelDepartment of Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, The Netherlands.
Sahar El AidyHost-Microbe Metabolic Interactions, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands. s.elaidy@uva.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPseudomonas fluorescens is a Gram-negative bacterium with a remarkable metabolic and physiological versatility that enables it to adapt and colonize diverse ecological niches, including the human small intestine. While serotonin is primarily found in high concentrations in gut tissue, its levels in the lumen can be elevated in conditions such as celiac disease, where P. fluorescens is also found in increased abundance. The potential effects of serotonin on P. fluorescens in such contexts remain unclear.

resultsWe demonstrate that P. fluorescens metabolizes serotonin primarily into 5-hydroxyindole-3-acetic acid (5-HIAA) and, to a lesser extent, into 5-hydroxytryptophol and N-acetylserotonin. Gene expression analysis revealed significant changes in oxidative stress-related pathways over time, and proteomic analysis confirmed the shifts seen particularly in amino acid catabolic pathways. Serotonin metabolism also enhanced bacterial resistance to oxidative stress, suggesting a protective role.

conclusionsThe findings reveal a novel mechanism by which serotonin modulates the metabolism and stress responses of P. fluorescens. This study provides insight into how P. fluorescens adapts to serotonin-rich environments, such as in celiac disease, and may inform future research on microbial interactions with host-derived metabolites in disease contexts.

Indexed as

Pseudomonas fluorescensSerotoninStress, PhysiologicalOxidative StressSerotonin5-HIAAAmino acids catabolismIndole metabolitePseudomonas fluorescensSerotonin

Identifiers

PMID41174663
PMCPMC12577022

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.