ArticleBMC infectious diseases2025
Detection of respiratory pathogens in infants using targeted next-generation sequencing versus conventional methods in Qingdao.
Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Decoding pulmonary infections through targeted next-generation sequencing: comprehensive pathogen profiling in 999 clinical cases.Frontiers in cellular and infection microbiology · 2026Article
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7 authors.
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Abstract
backgroundInfants are the most frequently hospitalized group for respiratory tract infections, and rapid and accurate pathogen identification is critical for effective management. However, conventional diagnostic methods often have limited sensitivity and a narrow detection range. This study aimed to compare the detection of respiratory pathogens in infants using targeted next-generation sequencing (tNGS) and conventional methods in Qingdao, China.
methodsThis study included infants aged 0-1 year with a respiratory infection (i.e., presenting with at least two of the following: cough, nasal congestion, sputum, sneezing, or dyspnea) admitted to Qingdao Women and Children's Hospital from March to May 2023. Bacterial culture, isothermal amplification, fluorescent PCR, and tNGS were performed to detect common pathogens.
resultsThe study included 95 patients. The highest positive rates were for the respiratory syncytial virus (RSV) (34/95, 35.8%), followed by influenza A virus (IAV) (17/95, 17.9%) and Acinetobacter baumannii (A. baumannii) (17/95, 17.9%). Single-pathogen infections accounted for 47.4%, and mixed infections for 47.4%. The positive detection rates for conventional methods and tNGS were 27.4% and 91.6%, respectively. The consistency rate between tNGS and conventional methods was 76.9%. There were significant differences between the two methods in the detection rates of viruses (P = 0.049) and bacteria (P = 0.016). The detection rate of pathogens using tNGS was higher than that with conventional methods (P = 0.023).
conclusionsRSV, IAV, A. baumannii, HRV, and S. pneumoniae are common pathogens in infants < 1 year old in Qingdao (China). tNGS is more sensitive and has a wider detection range than conventional methods. CLINICAL TRIAL NUMBER: Not applicable.
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