Evidence map›Paper›PMID 41174510›Full record

ArticleBMC plant biology2025

Molecular characterization of Tetragonia tetragonoides (Pall.) Aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress.

Lisha Cao, Fuying Xie, Lihua Chen, Zhengfeng Wang, Mei Zhang, Shuguang Jian

Abstract read
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lisha CaoGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Fuying XieGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Lihua ChenGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Zhengfeng WangGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Mei ZhangGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. zhangmei@scbg.ac.cn.
Shuguang JianGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. jiansg@scbg.ac.cn.

Funding

the Guangdong Science and Technology Program 2024B1212050007the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) GML2019ZD0408
6 · The paper itself

Abstract

Tetragonia tetragonoides (Pall.) Kuntze, a nutritional and medicinal secretohalophyte with excellent tolerance to environmental stresses, is widely distributed in the coastal tropics and subtropics worldwide. Aquaporin (AQP) proteins are water channel proteins that facilitate the transport of water and other substrates across cell membranes. AQPs play important roles in physiological processes, including water transport, nutrient acquisition (carbon, nitrogen, and micronutrients), and abiotic stress responses. In this study, we identified 58 candidate TtAQP genes in the T. tetragonoides genome and classified them into five subfamilies based on phylogenetic analysis with 15 plasma membrane intrinsic proteins (PIPs), 18 tonoplast intrinsic proteins (TIPs), 19 NOD26-like intrinsic proteins (NIPs), 4 small basic intrinsic proteins (SIPs) and 2 uncategorized X intrinsic proteins (XIPs). Gene structure and protein conserved domain analyses showed that the majority of the deduced TtAQPs contained signature transmembrane domains, NPA motifs, the ar/R selectivity filter, and Froger’s positions, suggesting substrate specificity for these TtAQPs. Analyses of cis-acting elements (CEs) in TtAQPs’ promoter regions revealed the presence of stress-responsive and hormone responsive elements, indicating complex regulatory mechanisms for the expression of TtAQPs. TtAQPs exhibited different expression patterns among tissues and under different stress conditions based on RNA sequencing and quantitative reverse transcription PCR assays. Several TtAQPs were cloned and heterologously expressed in yeast to confirm the stress tolerance conferred by the overexpression of these genes. This study provides a comprehensive framework for further functional studies of TtAQPs and corresponding reference for their potential applications in developing stress-tolerant transgenic plants.

Indexed as

AquaporinsHeat-Shock ResponsePlant ProteinsDroughtsGene Expression Regulation, PlantPhylogenySalinitySalt StressStress, PhysiologicalAquaporinsPlant ProteinsAbiotic stress tolerenceAquaporins (AQPs)Tetragonia tetragonoides

Identifiers

PMID41174510
PMCPMC12577117

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.