ArticleMethods in molecular biology (Clifton, N.J.)2026
Exploring the Functional Consequences of Protein-Coding Alternative Splice Variants at a Genome Scale: A Guide to Utilizing EXPANSION.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter serves as a comprehensive user guide for EXPANSION ( https://expansion.bioinfolab.sns.it/), an integrated web server dedicated to exploring the functional consequences of protein-coding alternative splice variants from genome-scale transcriptomics datasets. By amalgamating data from differentially expressed protein-coding transcripts, domain architecture, protein interaction networks, and gene enrichment analysis, EXPANSION provides researchers with a robust toolkit to dissect the impact of alternative splicing events on cellular functions. From navigating precalculated datasets to uploading custom data, visualizing transcript expression, scrutinizing splicing variations, and performing overrepresentation analysis, this chapter elucidates the key procedures involved in harnessing EXPANSION for a detailed exploration of protein alternative splicing from transcriptomics datasets in both healthy and disease conditions.
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