ArticleScientific reports2025
Isothermal nucleic acid amplification assays for the detection of porcine stool-associated RNA virus.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Pigs are a vital component of agricultural economies and a major source of livestock worldwide. The Porcine Stool-Associated virus (Posavirus), a newly identified member of the Picornavirales order, has been associated with enteric infections in swine. Recombinase Polymerase Amplification (RPA) and Polymerase Spiral Reaction (PSR), two isothermal amplification methods, were developed and optimized in this study to identify the posavirus in pig stool samples quickly and effectively. Primers that target the posavirus's polyprotein region were designed for both RPA and PSR assays, and reaction parameters were optimized. Sensitivity assessments revealed that the RPA assay had a detection limit of 5.34 × 10
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