ArticleThe EMBO journal2025
Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.
Article in The EMBO journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Brain organoids as models of extracellular vesicle-mediated human neural communication.Neural regeneration research · 2026Article
- Elucidating Gene Functions in Congenital Heart Disease.Current treatment options in cardiovascular medicine · 2026Review
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Authors and funding
12 authors.
Funding
Abstract
The role of long non-coding RNAs (lncRNAs) in early mammalian embryogenesis remains unclear due to the complexity of the regulatory mechanisms involving lncRNAs and the limited availability of embryo samples. Stem cell-based models, such as mouse gastruloids, provide new ways to address these challenges. Here, we investigate the function of ultra-conserved lncRNA T-UCstem1 in mammalian body plan formation. Combining morphological and immunofluorescence imaging analyses with bulk and single-cell transcriptomics, we provide evidence indicating that T-UCstem1 is important for mouse gastruloid development and anteroposterior axis extension. T-UCstem1 depletion in gastruloids results in their aberrant development, with defects in the expression of differentiation markers and persistence of pluripotency gene expression. Our single-cell analyses reveal higher levels of cellular heterogeneity in T-UCstem1-knockdown gastruloids. The presence of cell populations co-expressing pluripotency and differentiation markers points to an important role of T-UCstem1 in the establishment and maintenance of cell identity. Mechanistically, we show that T-UCstem1 acts in a non-cell-autonomous manner through regulation of the Dickkopf-related protein 1 (DKK1)-dependent WNT pathway. Our study identifies a new role for an ultra-conserved lncRNA in gastruloid development and highlights gastruloids as a model system for studying lncRNAs in early development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.