Evidence map›Paper›PMID 41174038›Full record

ArticleNature materials2026

Tumour priming by ultrasound mechanogenetics for CAR T therapy.

Chi Woo Yoon, Chunyang Song, Dung Ngo Minh Nguyen, Xi Yu, Linshan Zhu, Phuong Ho, Ziliang Huang, Gengxi Lu, Yuxuan Wang, Fan Wei and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature materials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Sono-Mechanogenetics: Linking Ultrasound Physics With Cellular Mechanobiology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  6. Review
  7. Article
  8. Review
  9. Oncology research · 2026
    Review
  10. Mechanomedicine: Present state and future promise.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Chi Woo YoonThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA. soulishguy@gmail.com.ORCID 0000-0003-4619-7083
Chunyang SongThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Dung Ngo Minh NguyenThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Xi YuThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0009-0004-5003-7298
Linshan ZhuThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Phuong HoThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Ziliang HuangThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.ORCID 0000-0002-4102-3300
Gengxi LuThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0001-7497-050X
Yuxuan WangThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Fan WeiThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.ORCID 0009-0003-0652-4841
Yunjia QuThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Ali ZamatThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Alexa LewisThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.ORCID 0009-0005-2595-3929
Ruimin ChenThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Yushun ZengThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0001-5995-4085
Priyankan DattaDepartment of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0002-2899-6714
Nan Sook LeeThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Christina JamiesonDepartment of Urology and Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA.
Bingfei YuDepartment of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0001-5550-6701
K Kirk ShungThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Qifa ZhouThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0003-1527-3020
Longwei LiuThe Alfred E. Mann Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA. longweil@usc.edu.ORCID 0000-0003-4229-9066
Yingxiao WangThe Shu Chien-Gene Lay Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA. ywang283@usc.edu.ORCID 0000-0003-0265-326X

Funding

Contemporary Approaches to Cancer Cell Signaling and CommunicationT32CA009523 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DONOGHUE, DANIEL J, YANG, JING · 1985 to 2025
$12.1M
Role of Spatiotemporal Epigenetic Dynamics in Regulating Endothelial Gene Expressions under FlowsR01HL121365 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHIEN, SHU, WANG, YINGXIAO · 2014 to 2025
$7.5M
Ultrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3M
Single Cell Tracking of 3D Epigenetic Landscape Evolution During Embryonic DevelopmentR01HD107206 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Sheng Zhong · 2022 to 2026
$3.2M
Acoustothermogenetics for Cell EngineeringR35GM140929 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI WANG, YINGXIAO · 2021 to 2025
$2.9M
Ultrasound-controlled remote activation of CAR T cells for localized tumor immunotherapyR01EB029122 · NIBIB · UNIVERSITY OF SOUTHERN CALIFORNIA · PI WANG, YINGXIAO · 2020 to 2023
$1.7M
Development of single fluorophore biosensors for multiplex imaging of CAR T SignalingK01EB035649 · NIBIB · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Longwei Liu · 2024 to 2026
$455k
NCI NIH HHS R01 CA262815NCI NIH HHS T32 CA009523NHLBI NIH HHS R01 HL121365NIBIB NIH HHS K01 EB035649NIBIB NIH HHS R01 EB029122NICHD NIH HHS R01 HD107206NIGMS NIH HHS R35 GM140929U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) HD107206U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) HL121365U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB) EB029122U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB) K01EB035649U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) GM140929
6 · The paper itself

Abstract

Cell-based cancer immunotherapy holds potential as a therapeutic approach, yet its application for solid tumour treatment remains challenging. Here we report a focused-ultrasound-based approach that mechanically induces the localized expression of CD19 antigen within a subpopulation of cells within solid tumours, which function as local 'training centres' to activate chimeric antigen receptor T cells. Activated chimeric antigen receptor T cells attack the whole cancer cell population near the tumour site, thus achieving cancer suppression. The system achieves targeted gene expression by integrating focused-ultrasound-triggered mechanical stimulation and the subsequent calcium response of cancer cells with a doxycycline-gated AND-logic genetic circuit, both of which need to be active for effective induction of CD19 expression. We validate the functionality of the approach in vitro, in organoids and in vivo, achieving direct control of user-designed gene expressions through FUS-mediated mechanical stimulation without the need of any cofactor, demonstrating the approach's potential as a versatile platform for precisely controllable immunotherapy. Overall, our combinatorial approach offers a focused-ultrasound-controlled remote and non-invasive priming of solid tumours for effective and safe chimeric antigen receptor T cell immunotherapy via the induced production of clinically validated antigens.

Indexed as

Immunotherapy, AdoptiveNeoplasmsReceptors, Chimeric AntigenT-LymphocytesUltrasonic WavesAnimalsAntigens, CD19Cell Line, TumorHumansMiceAntigens, CD19Receptors, Chimeric Antigen

Identifiers

PMID41174038
PMCPMC12867766

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.