ArticleNature communications2025
Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Spatially Encoded Protocell Network for Non-Cascaded Parallel Biocomputation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- DNA-Based Boolean Logic Gates for Molecular Computation and Biosensing: A Critical Review.Molecular biotechnology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
In this work, we establish the use of nucleic acid hybridization to program the activation of catalysis in enzymes. We do this via "thiol switching", using thiolated oligonucleotides: a protein is inactivated by conjugation to an oligonucleotide via a disulfide linkage; hybridization of the thiolated complementary oligonucleotide ensues disulfide exchange, the liberation of the enzyme, and the activation of enzymatic catalysis. In doing so, we couple the most specific recognition event (hybridization) to the most effective tool of signal amplification (catalysis). As potential applications, we illustrate the use of hybridization-activated catalysis for programming the specificity of activation for one specific enzyme and engineering of Boolean logic elements. For biosensing, we illustrate the sequence-specific detection of a target oligonucleotide at a nanomolar level, in the presence of gram quantities of genomic nucleic acids, using simple mobile phone camera. We anticipate that this technology will find use in diverse areas of biotechnology and biomedicine.
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Registered trials
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