ArticleScientific data2025
Genome assembly and whole-genome resequencing study of Butuo Black sheep (Ovis aries).
Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Genome assembly and whole-genome resequencing study of Butuo Black sheep (Ovis aries).Scientific data · 2025Article
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9 authors.
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Abstract
Butuo Black sheep (BBS), an ancient indigenous Chinese breed, has co-evolved with the Yi people's semi-nomadic lifestyle and demonstrated exceptional adaptability to high-altitude migrations. However, a high-quality reference genome for BBS is still lacking. In this study, we established a high-quality chromosome-level genome assembly of BBS using PacBio HiFi sequencing. The final assembled genome size was approximately 2.95 Gb, with a contig N50 of 71.45 Mb and a scaffold N50 of 92.26 Mb. The genome assembly achieved a high Benchmarking Universal Single-Copy Orthologs (BUSCO) score of 95.9%, indicating its high completeness and quality. The de novo genome prediction revealed that repetitive sequences accounted for 47.74% of the genome, with long interspersed nuclear elements (LINEs) being the most abundant. Additionally, we present 50 BBS shotgun genomes sequenced using the Illumina HiSeq 2000 platform, with a mean coverage of 10.36×. The study generated approximately 1.2 terabytes of raw data, with 99.9% clean reads mapping successfully to the sheep reference genome at 99.6% coverage. This extensive dataset provides a valuable resource for studying genetic diversity and evolutionary patterns in BBS.
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