Evidence map›Paper›PMID 41173327›Full record

ArticleVirus research2025

Isolation and characterization of a novel lytic bacteriophage vB_EcoS_P78 against multidrug-resistant Escherichia coli.

Rong Wen, Jiahao Tong, Shoude Liu, Chengbo Zheng, Jinshui Zheng, Donghai Peng, Ming Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rong WenNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Jiahao TongNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Shoude LiuNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Chengbo ZhengNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Jinshui ZhengNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Donghai PengNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
Ming SunNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China. Electronic address: m98sun@mail.hzau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Escherichia coli phage vB_EcoS_P78, a novel bacteriophage isolated from swine feces, exhibits typical siphovirus morphology with an isometric head and a non-contractile tail, forming clear plaques approximately 4 mm in diameter. It demonstrated a broad host range, lysing 75 % of tested ESBL-producing and MDR E. coli clinical isolates across multiple serogroups. The phage displayed strong stability, retaining activity at temperatures up to 50 °C and within a pH range of 3-10. Results from one-step growth assays showed a 20-minute latent period and that each infected cell produced a burst size of 220 PFU. Whole-genome sequencing revealed a 44,574 bp dsDNA genome containing 58 ORFs, with no antibiotic resistance, and virulence genes detected. Phylogenetic analysis based on intergenomic similarity and core protein phylogeny consistently supported its classification within the genus Dhillonvirus, showing 92.8 % similarity to Escherichia phage JL1. These results indicate that vB_EcoS_P78 represents a novel species of Dhillonvirus with potential therapeutic applications against multidrug-resistant E. coli.

Indexed as

ColiphagesEscherichia coliSiphoviridaeAnimalsDNA, ViralDrug Resistance, Multiple, BacterialEscherichia coli InfectionsFecesGenome, ViralHost SpecificityOpen Reading FramesPhylogenySwineWhole Genome SequencingDNA, ViralBacteriophageDiarrheaEscherichia coliGenomic characteristicsMultidrug-resistant (MDR)Siphovirus

Identifiers

PMID41173327
PMCPMC12677095

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.