ArticleJournal of advanced research2026
Functional divergence of BnaWRKY7 homologs drives phytosterol variations in polyploid Brassica napus.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionGene duplication and subsequent functional divergence drive species evolution, adaptability, and biodiversity, particularly in polyploids. The polyploid Brassica napus has abundant phytosterols, which are crucial for plant growth and development, and human health. However, the regulatory mechanism of phytosterol biosynthesis remains poorly understood in polyploid systems.
objectivesThis study aims to provide a novel method for analyzing the functions of multi-copy homologous genes and to dissect the genetic and molecular basis of phytosterol biosynthesis in B. napus.
methodsGenome-wide association studies (GWAS), temporal transcriptome and phytosterol profiles were employed to identify phytosterol-regulatory genes. Transgenic validation (B. napus) combined with yeast one-hybrid assay, dual-luciferase reporter assay and electrophoretic mobility shift assay was used to reveal phytosterol biosynthesis mechanisms.
resultsThrough dynamic gene expression analysis and phytosterol profiles, 103 transcription factors (TFs) were preliminarily screened out by coordinating an intricate transcriptional program. Then, GWAS revealed two highly significant loci on chromosomes A03 and C07, located in syntenic regions between the A
conclusionIn this study, we established a multi-copy gene co-screening strategy and elucidated the molecular mechanism. BnaWRKY7 regulates phytosterol variations by interacting with four key phytosterol biosynthesis genes. The findings enhance our understanding of the temporal regulation of phytosterols in polyploids, establish a foundation for future research on duplicated genes and genome evolution, and propose targets for high-phytosterol crops.
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