ArticleBrazilian dental journal2025
Effect of sugarcane cystatin CaneCPI-5 on osteogenic differentiation of human dental pulp cells.
Article in Brazilian dental journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Casearia sylvestris Derivatives Inhibit Inflammation and Oxidative Activity and Promote Healing in Human Gingival Fibroblasts - An In Vitro Study.Oral health & preventive dentistry · 2026Article
- Maqui berry cystatin inhibits cathepsin k activity and stimulates osteogenic differentiation of human dental pulp cells.Journal of applied oral science : revista FOB · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For pulp and periapical repair, or endodontic regeneration to occur, it is necessary for mesenchymal stem cells from the apical papilla and/or dental pulp to proliferate, migrate to the site of injury, and differentiate into cells that produce mineralized tissue. Therefore, materials used in endodontic therapy should stimulate those events. Phytocystatins are plant-derived cystatins capable of inhibiting cathepsins. Some of them are produced recombinantly, such as CaneCPI-5 (derived from sugar cane). Considering their pro-osteogenic potential, this study aimed to assess the cytocompatibility and effect of CaneCPI-5 on the proliferation, migration, and osteogenic differentiation of human dental pulp stem cells (hDPSCs). The hDPSCs exposed to CaneCPI-5 and unexposed (control) were evaluated as follows: cell viability, by alamarBlue assay; proliferation, by bromodeoxyuridine (BrdU) incorporation assay; migration, by transwell assay; deposition of mineralized nodules, by alizarin red staining; and activity of tissue-nonspecific alkaline phosphatase (TNAP). Data were evaluated by one- or two-way ANOVA, followed by Tukey or Kruskal-Wallis post-test and Dunn or Mann Whitney post-test. CaneCPI-5 was cytocompatible and induced higher migration, proliferation, formation of mineralized nodules, and TNAP activity in hDPSCs compared to control. In conclusion, CaneCPI-5 represents a molecule with promising application in endodontic therapy to stimulate events necessary for pulp and periapical repair/regeneration.
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Registered trials
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