Evidence map›Paper›PMID 41172135›Full record

ArticleJournal of the American Society for Mass Spectrometry2025

Rapid Glass Wool Enrichment of Glycopeptides for MALDI-MS Analysis of Immunoglobulin G Glycosylation in COVID-19 Samples.

Yuye Zhou, Felicia Karlahag, Sophia Schedin Weiss, Sara Jamshidi, Lars Tjernberg, Åsa Emmer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuye ZhouSchool of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, KTH Royal Institute of Technology, 10044 Stockholm, Sweden.ORCID 0000-0002-0186-7795
Felicia KarlahagSchool of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, KTH Royal Institute of Technology, 10044 Stockholm, Sweden.
Sophia Schedin WeissDivision of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Karolinska Institutet, 17164 Solna, Sweden.
Sara JamshidiSchool of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, KTH Royal Institute of Technology, 10044 Stockholm, Sweden.
Lars TjernbergDivision of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Karolinska Institutet, 17164 Solna, Sweden.
Åsa EmmerSchool of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, KTH Royal Institute of Technology, 10044 Stockholm, Sweden.ORCID 0000-0002-3444-9987

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glycosylation is the most common protein post-translational modification, affecting protein properties and functions. Abnormal variations in glycosylation are associated with diseases, e.g., coronavirus disease COVID-19. Matrix-assisted laser desorption/ionization mass spectrometry has been widely utilized for studying protein glycosylation, after proper purification of glycopeptides or glycans using hydrophilic interaction liquid chromatography (HILIC) or laboratory-synthesized hydrophilic materials. Here, glass wool tips were developed to enrich immunoglobulin G glycopeptides and applied in the analysis of COVID-19 patient samples as a proof-of-concept. A significant decrease in galactosylation was detected in the COVID-19 patient plasma sample compared to the reference sample. The tips developed in this work provided a cheap and simple enrichment alternative to commercial HILIC tips for studying protein glycosylation.

Indexed as

COVID-19GlycopeptidesImmunoglobulin GSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationGlassGlycosylationHumansSARS-CoV-2GlycopeptidesImmunoglobulin G

Identifiers

PMID41172135
PMCPMC12679630

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.