Evidence map›Paper›PMID 41170999›Full record

ArticlemBio2025

Experimental evolution of gene essentiality in bacteria.

Liang Bao, Zan Zhu, Ahmed Ismail, Bin Zhu, Vysakh Anandan, Marvin Whiteley, Todd Kitten, Ping Xu

Abstract read
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Specific host translation perturbations promote TnbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. A genome-widebioRxiv : the preprint server for biology · 2026
    Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Liang BaoDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.ORCID 0000-0002-0228-6124
Zan ZhuDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Ahmed IsmailDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Bin ZhuMassey Cancer Center, Virginia Commonwealth University, Richmond, Virginia, USA.
Vysakh AnandanDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Marvin WhiteleySchool of Biological Sciences, Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA.
Todd KittenDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.ORCID 0000-0001-6097-7583
Ping XuDepartment of Oral and Craniofacial Molecular Biology, Philips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.ORCID 0000-0002-8368-801X

Funding

Oral Streptococcal Fitness And Virulence genesR01DE030121 · NIDCR · VIRGINIA COMMONWEALTH UNIVERSITY · PI KITTEN, TODD O., XU, PING · 2021 to 2025
$2.5M
Comparative Experimental Evolution of Gene Essentiality in Streptococcus sanguinis and S. mutansR03DE034511 · NIDCR · VIRGINIA COMMONWEALTH UNIVERSITY · PI BAO, LIANG · 2025 to 2025
$311k
NIDCR NIH HHS R01 DE030121NIDCR NIH HHS R03 DE034511
6 · The paper itself

Abstract

Essential gene products carry out fundamental cellular activities in interaction with other components. However, the lack of essential gene mutants and appropriate methodologies to link essential gene functions with their partners poses significant challenges. We report here the generation in

Indexed as

Directed Molecular EvolutionEvolution, MolecularGenes, EssentialStreptococcus sanguisGene DeletionGenome, BacterialMutationWhole Genome Sequencingexperimental evolutionF1Fo-ATPase/V1Vo-ATPase/TrkA1-H1 gene pathwaygene essentialityspontaneous mutationsStreptococcuswhole-genome sequencing

Identifiers

PMID41170999
PMCPMC12691585

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.