ArticleIranian journal of veterinary research2025
Molecular detection and genotyping of
Article in Iranian journal of veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Equine piroplasmosis represents one of the main and serious health problems affecting the equines industry globally, caused by a tick-borne protozoa called Methods: We collected 116 apparently healthy horses from the study area during the first half of 2019 to identify Results: Through sequencing and phylogenetic analysis, our samples were grouped into clusters corresponding to genotype A and genotype E. Our results demonstrated that the qPCR had the highest sensitivity (100%) followed by conventional PCR (68%) while microscopic examination had the lowest sensitivity (38%). Furthermore, the negative predictive value (NPV) of qPCR was the highest (100%) compared with conventional PCR and microscopical examination (80.49% and 68.04%, respectively) which revealed that negative cases detected by qPCR were certainly correct compared with the other two diagnostic assays. Conclusion: It is highly recommended to incorporate PCR diagnostic assays alongside microscopic examination to evaluate the epidemiological status of equine piroplasmosis. Also, our study demonstrated that
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