ArticleEye banking and corneal transplantation2025
Feasibility of Descemet membrane endothelial keratoplasty with preloaded and trifolded graft after 96-hour storage and shipping.
Article in Eye banking and corneal transplantation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Purpose: Descemet membrane endothelial keratoplasty (DMEK) donor grafts can be safely trifolded, preloaded, and shipped for up to 96 hours without significant endothelial cell loss; however, clinical outcomes of such storage times have yet to be reported in human patients. Here we report a case of a trifolded, preloaded DMEK graft stored for 96 hours and transplanted in a 68-year-old female with Fuchs endothelial dystrophy. Methods: The DMEK graft was preloaded and trifolded with post-processing endothelial cell density (ECD) of 2558 cells/mm. Conclusions: We demonstrate feasibility of DMEK with a preloaded and triolded graft stored for 96 hours, with resolution of corneal edema. These results underscore the viability of international shipment and optimized resource distribution of donor tissue where there is a high global need for DMEK grafts.
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