Evidence map›Paper›PMID 41168776›Full record

ArticleMicrobiome2025

An innovative strategy for overcoming ultra-high ammonia nitrogen inhibition on anaerobic methanogenesis via stepwise domestication.

Heng Wu, Taili Dong, Anjie Li, Jing Chen, Huaiwen Zhang, Hongyi Lv, Caiyun Yang, Xiaohui Guo, Xuanmin Yang, Ling Qiu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Heng Wu *College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Taili Dong *Shandong Min-He Biotechnology Co Ltd, Penglai, 265600, China.
Anjie LiCollege of Grassland and Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Jing ChenCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Huaiwen ZhangCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Hongyi LvCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Caiyun YangCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Xiaohui GuoCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Xuanmin YangCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Ling QiuCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Changlin MiaoCollege of Landscape Architecture and Art, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Yiqing YaoCollege of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. dzhtyao@nwafu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe world is facing both an increasingly severe energy crisis and a growing problem of agricultural pollution. The utilization of agricultural waste by anaerobic digestion (AD), has received increasing attention. AD using representative waste cow dung results in total ammonia nitrogen (TAN) accumulation and inhibition of methanogens resulting in reduced CH

resultsIn this study, an incremental approach to gradually increasing the TAN concentration has been used for overcoming the problem. The results showed that at an ultra-high concentration of 6124.09 mg/L TAN, a 48-day domesticated AD system functioned stably and the cumulative CH

conclusionsStepwise increase the TAN concentration is a novel method that was demonstrated to be a practical and sustainable way to overcome ultra-high TAN inhibition (6124.09 mg/L). During domestication, the two-component system may regulate the microbial collaborative network to ensure microbial activity and high abundance enrichment, thus potentially constructing a methanogenic system dominated by hydrogenotrophic and acetoclastic methanogenesis, holding a promising application prospect. This study helped recognize the potential of methanogens in tolerating ultra-high inhibition and developed an achievable AD technology for robustly treating fecal residue and wastewater in practice.

Indexed as

AmmoniaMethaneNitrogenAnaerobiosisAnimalsBacteriaBioreactorsCattleDomesticationManureMetagenomicsMethanosarcinaAmmoniaManureMethaneNitrogenAmmonia inhibitionAnaerobic digestionEngineering application potentialEnvironmental microbiomeMethanogenesisStepwise domestication

Identifiers

PMID41168776
PMCPMC12574047

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.