ArticleAnnals of the New York Academy of Sciences2025
Mycoplasma agassizii Multilocus Sequence Typing Using Nanopore Sequencing: Insights Into Genetic Diversity and Isolate Characterization.
Article in Annals of the New York Academy of Sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Mycoplasma agassizii Multilocus Sequence Typing Using Nanopore Sequencing: Insights Into Genetic Diversity and Isolate Characterization.Annals of the New York Academy of Sciences · 2025Article
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Authors and funding
10 authors.
Funding
Abstract
Mycoplasma agassizii (Mycoplasmopsis agassizii) is a major pathogen responsible for upper respiratory tract disease (URTD) in tortoises, contributing to worldwide population declines. Despite its significance, tools for strain-level identification and epidemiological tracking remain limited. This study proposes a Multilocus Sequence Typing (MLST) scheme based on eight housekeeping genes (uvrB, tpiA, gyrB, efTu, rpoB, uvrA, gmK, and atpG), combined with Nanopore sequencing, to characterize M. agassizii isolates from tortoises in Italy and Portugal. Among 127 samples, 73 (57.5%) tested positive for M. agassizii. Analysis of 15 isolates revealed 14 distinct sequence types (STs). High genetic diversity was observed, even among samples from the same rescue centers. No correlation was found between STs and geographic origin or tortoise species. Four loci (uvrB, gyrB, rpoB, and gmK) were sufficient to discriminate all STs at a haplotype diversity cut-off ≥ 0.83. The standardized index of association (I
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