ArticleSTAR protocols2025
Protocol for double-crosslinking ChIP-seq to improve data quality and enhance detection of challenging chromatin targets.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- CEBPG demonstrates oncogene functions and modulates the expression of genes involved in cervical cancer related pathways.Translational cancer research · 2026Article
- Comparative analyses of ChIP-seq, CUT&RUN and CUT&Tag for Polycomb chromatin profiling.BMB reports · 2026Article
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin factors drive genome function, yet many lack direct DNA-binding activity and cannot be conventionally profiled. Here, we present dxChIP-seq, a double-crosslinking chromatin immunoprecipitation sequencing (ChIP-seq) protocol that improves mapping of chromatin factors, including those that do not bind DNA directly, while enhancing signal-to-noise ratio. We describe steps for double-crosslinking, focused ultrasonication, immunoprecipitation, DNA purification, and library preparation. We then detail procedures for sequencing and analysis. This protocol is compatible with adherent cells and complex multicellular structures. For complete details on the use and execution of this protocol, please refer to Cacioppo et al.
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Registered trials
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