Evidence map›Paper›PMID 41166034›Full record

ArticleApplied biochemistry and biotechnology2025

High-Level Expression of Humicola insolens Cutinase in Bacillus subtilis.

Linhui Li, Ruyu Sun, Ying Wang, Bo Xu, Lei Xue, Lingqia Su

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Linhui LiSchool of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, China.
Ruyu SunSchool of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, China.
Ying WangChina Tobacco Shanxi Industrial Co., Ltd, Xi'an, China.
Bo XuZhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
Lei XueChina Tobacco Shanxi Industrial Co., Ltd, Xi'an, China. xuelsky@163.com.
Lingqia SuSchool of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, China. sulingqia@jiangnan.edu.cn.ORCID http://orcid.org/0000-0003-2754-3964

Funding

Fundamental Research Funds for the Central Universities 5812050205226750National Natural Science Foundation of China 22478155Shandong Provincial Key Laboratory of Eco-environmental Science for Yellow River Delta DYRC20200205the Taishan Industrial Experts Program tscx202306145
6 · The paper itself

Abstract

Cutinase (EC 3.1.1.74), a serine esterase that degrades a variety of ester substrates into fatty acids, has significant potential for various applications. Bacillus subtilis is a food-safe strain known for its exceptional protein secretion capabilities. This study aims to enhance the expression of cutinase from Humicola insolens (HIC) in B. subtilis. Specifically, B. subtilis WS9 was selected as the expression host, with pHY300PLK as the expression vector, achieving an initial enzymatic activity of 35.00 U·mL

Indexed as

Bacillus subtilisCarboxylic Ester HydrolasesGene ExpressionBacterial ProteinsFermentationPromoter Regions, GeneticRecombinant ProteinsBacterial ProteinsCarboxylic Ester HydrolasescutinaseRecombinant ProteinsBacillus subtilisChaperone proteinFermentation optimizationHumicola insolens cutinasePromoter

Identifiers

PMID41166034

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.