Evidence map›Paper›PMID 41165893›Full record

ArticleFish physiology and biochemistry2025

Dietary starch levels modulate growth performance, glucose utilization, and lipogenesis in mandarin fish (Siniperca chuatsi).

Zhihao Han, Shiwen Chen, Ye Gong, Shengchao Liu, Zeqiang Sun, Fan Gao, Naisong Chen, Xuxiong Huang, Haitao Zhang, Songlin Li

Abstract read
PubMed Publisher
In one paragraph

Article in Fish physiology and biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhihao HanMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Shiwen ChenMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Ye GongMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Shengchao LiuMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Zeqiang SunMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Fan GaoMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Naisong ChenMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Xuxiong HuangMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Haitao ZhangKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture and Rural Affairs, Zhanjiang, 524000, China.
Songlin LiMinistry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China. slli@shou.edu.cn.

Funding

Shanghai Agricultural Science and Technology Innovation Program K2024-02-08-00-12-F00029Shanghai Sailing Program 24YF2717200
6 · The paper itself

Abstract

The study investigated how varying levels of dietary starch influence the growth, health status, and glucose-lipid metabolism of mandarin fish (initial body weight: 106 ± 1 g). Five diets containing graded levels of digestive starch were formulated (6.56%, 9.03%, 13.03%, 16.46%, and 19.57%), designated as D6.56, D9.03, D13.03, D16.46, and D19.57, respectively. Each diet was administered to triplicate groups of mandarin fish twice daily over 8 weeks. Results showed that growth performance increased linearly, peaking in the D13.03 group starch level before declining (P < 0.05). Liver and muscle lipid content and hepatic glycogen increased significantly in a quadratic and linear manner, respectively (P < 0.05). Transcriptional analysis revealed a starch-dependent upregulation of insulin signaling (ira, pi3kr1, and akt1) and glycolysis (gk, pfkl, and pk) genes, with concurrent downregulation of gluconeogenesis (fbp1 and g6pc) (P < 0.05). Protein expression analysis showed the ratio of p-PI3KR1 to PI3KR1 was significantly elevated as dietary starch increased, with the D13.03 group showing higher ratios than the D19.57 group (P < 0.05). FoxO1 expression was decreased in high-starch groups and reached a peak in the D13.03 group, while the phosphorylation of FoxO1 was significantly elevated (P < 0.05). Additionally, ChREBP and SREBP-1 protein levels were elevated in the D13.03 and D19.57 groups compared to the D6.56 groups (P < 0.05). In conclusion, mandarin fish showed superior carbohydrate tolerance with optimal growth at 13.03% dietary starch, which was partly due to the superior capacity to convert carbohydrates into triglycerides.

Indexed as

Dietary CarbohydratesFishesGlucoseLipogenesisPerciformesStarchAnimal FeedAnimalsDietLipid MetabolismLiverDietary CarbohydratesGlucoseStarchCarbohydrate utilizationGlucose metabolismLipogenesisMandarin fishStarch level

Identifiers

PMID41165893

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.