Evidence map›Paper›PMID 41165868›Full record

ArticleMikrochimica acta2025

Simultaneous recognition of two MicroRNAs associated with early stages of lung cancer by DNA hairpin-templated fluorescent nanoclusters.

Sheida Zoughi, Farnoush Faridbod, Saeed Mohebbi, Sharif Moradi

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sheida ZoughiAnalytical Chemistry Department, Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
Farnoush FaridbodAnalytical Chemistry Department, Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran. faridbodf@ut.ac.ir.
Saeed MohebbiDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Sharif MoradiDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. sh.moradi@royan-rc.ac.ir.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

miRNA-21-5p and miRNA-155-5p have emerged as two promising biomarkers in body fluids during the early stages of lung cancer. This study focused on designing a dual-target optical biosensor for the simultaneous detection of miRNA-21 and miRNA-155. DNA hairpin probes and metal nanoclusters are the materials used in this design. Light-emitting gold nanoclusters (AuNCs) and copper-silver nanoclusters (Cu/AgNCs) were located on hairpin probes to achieve fluorescence-based detection. The biosensor leverages the distinct fluorescence properties of these nanoclusters to detect target miRNAs (miRNA-21 enhances the emission of Cu/AgNCs, while miRNA-155 quenches the emission of AuNCs). The biosensor demonstrated remarkable sensitivity toward miRNA-21 and miRNA-155. Detection limits were 1 pM and 5 pM, and linear response ranges were 15-140 pM and 15-125 pM for miRNA-21 and miRNA-155, respectively. The response of the nanobiosensor was validated by RT-qPCR as the gold standard method in analyses of miRNAs in human serum samples and lung cancer cells. The obtained results confirmed the accuracy and reliability of the biosensor. Compared to existing biosensors, the proposed design avoids complex surface modifications and enzyme usage, ensuring cost-effectiveness and operational simplicity.

Indexed as

Biosensing TechniquesFluorescent DyesLung NeoplasmsMetal NanoparticlesMicroRNAsCopperDNADNA ProbesGoldHumansLimit of DetectionSilverSpectrometry, FluorescenceCopperDNADNA ProbesFluorescent DyesGoldMicroRNAsMIRN155 microRNA, humanMIRN21 microRNA, humanSilverBimetallic nanoclustersDual-target detectionEnzyme-free biosensorsFluorescence detectionLung cancer diagnosisMicroRNA-155MicroRNA-21

Identifiers

PMID41165868

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.