Evidence map›Paper›PMID 41165773›Full record

ArticleCell and tissue research2025

The antioxidant, N-acetyl-L-cysteine, affects beta cell oxidative stress, insulin secretion, and intracellular signaling pathways in MIN6 cells.

Meg Schuurman, Rachel B Wilson, Nica Borradaile, Rennian Wang

Abstract read
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Article in Cell and tissue research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Meg SchuurmanChildren's Health Research Institute, London, ON, N6C 2V5, Canada.
Rachel B WilsonChildren's Health Research Institute, London, ON, N6C 2V5, Canada.
Nica BorradaileDepartment of Physiology & Pharmacology, University of Western Ontario, London, ON, N6A 3K7, Canada.
Rennian WangChildren's Health Research Institute, London, ON, N6C 2V5, Canada. rwang@uwo.ca.ORCID http://orcid.org/0000-0002-6109-5029

Funding

CIHR 152944)
6 · The paper itself

Abstract

There is intense public interest in the potential of antioxidant supplements as a preventative treatment for conditions associated with increased oxidative stress, such as type 2 diabetes mellitus. However, there is limited evidence regarding the effects of antioxidants on beta cells during physiological and pathological conditions. We examined the direct effects of N-acetyl-L-cysteine (NAC) supplementation on the MIN6 beta cell line under physiological and fatty acid stress conditions. MIN6 cells were cultured in growth medium with or without NAC (physiological conditions) or growth medium plus palmitate with or without NAC (fatty acid stress conditions). We observed that MIN6 cells receiving NAC, under physiological or fatty acid stress conditions, displayed significantly reduced cellular ATP content and oxidative stress without changes to markers of cell proliferation or apoptosis. Regardless of the treatment conditions, NAC lowered basal insulin release with no change to cellular insulin content, yet improved high glucose-stimulated insulin secretion (GSIS) was only observed under physiological conditions. Importantly, phosphorylated AKT was significantly reduced with NAC supplementation under physiological and fatty acid stress conditions, which was inversely proportional to ERK1/2

Indexed as

AcetylcysteineAntioxidantsInsulinInsulin-Secreting CellsOxidative StressSignal TransductionAdenosine TriphosphateAnimalsCell LineGlucoseInsulin SecretionMicePhosphorylationProto-Oncogene Proteins c-aktAcetylcysteineAdenosine TriphosphateAntioxidantsGlucoseInsulinProto-Oncogene Proteins c-aktBeta-cell ATP contentInsulin secretion and GSISN-acetyl-L-cysteine (NAC)

Identifiers

PMID41165773

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.