ArticleStem cell research & therapy2025
Microgravity-driven Rab27B activation amplifies mesenchymal stem cell-derived extracellular vesicle production and functions.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Beyond Passage Numbers: How Culture Conditions and Population-Doubling Metrics Reporting Shape the Quality of Umbilical Cord-Derived MSCs and Extracellular Vesicles.International journal of molecular sciences · 2026Review
- Harnessing MSCs plasticity: mechanisms and determinants of bidirectional immune regulation in stem-cell-based immunotherapy.Stem cell research & therapy · 2026Review
- Tissue regeneration strategies based on mesenchymal stem cell-derived extracellular vesicles: from bench to bedside.Burns & trauma · 2026Review
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Authors and funding
6 authors.
Funding
Abstract
backgroundMesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are emerging as promising treatments for immunomodulation and tissue regeneration. However, the scalable production of functionally enhanced EVs remains a critical challenge. This study introduces microgravity culture using a three-dimensional (3D) rotating cell culture system as a novel strategy to optimize MSC-EVs yield and bioactivity.
methodsWe first investigated the effects of microgravity on the proliferation and stemness of human umbilical cord-derived MSCs (UCMSCs). The yield of microgravity-derived EVs (µg-EVs) was quantified by nanoparticle tracking analysis. The function of µg-EVs was analyzed by proteomic profiling and further assessed by macrophage polarization and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Proteomic analysis of UCMSCs was performed to further explore the underlying mechanisms of EVs biogenesis and functional activity under microgravity condition.
resultsMicrogravity culture significantly enhanced UCMSCs proliferation and stemness. Compared with conventional static culture, EVs production increased by 7.7-fold under microgravity. Functionally, µg-EVs more effectively promoted macrophage polarization toward the anti-inflammatory M2 phenotype and significantly enhanced the osteogenic differentiation capacity of PDLSCs. Mechanistically, Rab27B upregulation in microgravity-cultured UCMSCs was associated with increased EVs secretion and enhanced therapeutic efficacy.
conclusionsThis study identifies microgravity as an effective platform for the large-scale production of high-quality UCMSC-EVs, addressing key manufacturing barriers and accelerating the clinical translation of EVs-based therapies.
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