ArticleScientific reports2025
Proteome of seminal plasma and sperm associated with sperm survival following cryopreservation in the Red Wolf (Canis rufus).
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Seasonal Changes in the Seminal Plasma Proteome of the Crab-Eating Fox (Journal of proteome research · 2026Article
- Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding of the impacts of seminal plasma and sperm proteins on both animal and human fertility has expanded rapidly in the last decade. However, little is known about the reproductive proteomes of endangered species. In this study, we characterized for the first time the seminal plasma and sperm proteomes of the critically endangered Red Wolf and evaluated differences in proteomic signatures between ejaculates which experienced "High" versus "Baseline" (i.e. population average) sperm cryo-resilience. Highly cryo-resilient ejaculates (High_CR, n = 5) maintained > 50% sperm total motility relative to fresh samples following cryopreservation and post-thaw incubation, as well as acrosome integrity post-thaw. These samples had significantly higher expression of A1BG, APBB1, KRT1, KRT10, LOC609402 and LOC100685620 (AGP) proteins in seminal plasma, and significantly reduced expression of RHOA, NUP62, SMYD4, ARHGD1B, CAPG, CSTB, and CFL1 proteins in sperm compared with Baseline cryo-resilient samples (Base_CR, n = 5). Differences in protein expression indicated that alterations in physiological pathways including blood clotting cascades (seminal plasma) and both actin dynamics and immune pathways (sperm) are associated with sperm cryo-resilience. This new knowledge may prove useful in future optimization of semen cryopreservation and assisted reproductive technologies for the critically endangered Red Wolf.
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