ArticleScientific reports2025
Optimised reverse-phase chromatography for clinical detection of vitamins A and E in serum.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Vitamin A and E are indispensable fat-soluble vitamins that play pivotal roles in promoting human health and well-being. Accurate quantification of these vitamins in biological samples is critical. Thus, we aim to develop and optimise a sensitive method suitable for simultaneous analysis of vitamin A and E. We introduced a method for simultaneous detection of Vitamin A and E using reverse-phase high-performance liquid chromatography (RP-HPLC). Calibration curves and quality control samples were prepared using a 4% of Bovine Serum Albumin (BSA) matrix. The analytes and internal standard (ISTD), Dodecanophenone were effectively separated using a polar column and detected via UV detector at wavelengths of 255 nm, 298 nm, and 325 nm respectively, with a total run time of 30 min. Results: The method demonstrated excellent linearity with regression coefficients (r²) > 0.995. The optimisation process of the HPLC method affirmed its precision and reliability, meeting analytical chemistry standards. Subsequently, this validated method was applied to serum samples obtained from patients and a population cohort exhibiting distinct clinical pathologies, including individuals without colorectal cancer (CRC), those with symptomatic and asymptomatic CRC. Conclusions: In conclusion, this HPLC method offers a reliable means for routine quantification of vitamin A and E in human serum, holding promise for enhancing nutritional research and clinical diagnostics. Its novelty lies in the use of a surrogate BSA matrix with a polar RP-HPLC column and multi-wavelength detection, enabling robust quantification of endogenous analytes in clinically relevant cohorts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.