Evidence map›Paper›PMID 41162450›Full record

ArticleScientific reports2025

Real-time PCR assay design and validation for Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, bacteria associated with increased HIV susceptibility.

Sydney G Nelson, Megan Spyers-Duran, Maliha Aziz, Danielle Marcone, Deneen Nault, Gizachew Demessie, Rupert Kaul, Ronald M Galiwango, Rebecca Yee, Cindy M Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sydney G NelsonAntibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, 20052, USA.
Megan Spyers-DuranAntibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, 20052, USA.
Maliha AzizAntibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, 20052, USA.
Danielle MarconeAntibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, 20052, USA.
Deneen NaultGeorge Washington University Hospital, Washington, DC, 20037, USA.
Gizachew DemessieGeorge Washington University Hospital, Washington, DC, 20037, USA.
Rupert KaulDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Ronald M GaliwangoRakai Health Sciences Program, Kalisizo, Uganda.
Rebecca YeeGeorge Washington University School of Medicine and Health Sciences, Washington, DC, 20052, USA.
Cindy M LiuAntibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, 20052, USA. cindyliu@gwu.edu.

Funding

How sex, host microenvironment, and immune responses shape acquisition of genital bacteria that increase HIV riskR01DK131936 · NIDDK · GEORGE WASHINGTON UNIVERSITY · PI Cindy Liu · 2022 to 2026
$3.0M
CIHR PJT-180629FIC NIH HHS 4D43TW009578-04NIH HHS R01DK131936
6 · The paper itself

Abstract

Many genital anaerobes, including Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, are underexplored despite being Bacteria Associated with HIV Seroconversion, Inflammation, and immune Cells (BASICs). This is partly due to the lack of molecular tools for their detection and quantification. To address this gap, we designed and validated three real-time qPCR assays for rapid and cost-effective analysis. qPCR assays were designed based on the species-specific core genomes of the three target genital anaerobic species. Assay sensitivity, specificity, and quantification characteristics were assessed using a synthetic oligonucleotide and DNA extracted from closely related species (n = 27-42) and human urogenital swabs (n = 111-114). The resultant assays demonstrated 100% sensitivity and specificity for bacterial isolates and high sensitivity (94.6%-97.7%) and specificity (92.8%-95.7%) for human urogenital swabs. The linear dynamic range was 5.0 × 10

Indexed as

BacillotaGram-Positive Bacterial InfectionsHIV InfectionsPeptostreptococcusPrevotellaReal-Time Polymerase Chain ReactionBacteroidaceae InfectionsDNA, BacterialFemaleHumansMaleSensitivity and SpecificityDNA, BacterialBacteria associated with HIV seroconversionBacterial vaginosisDialister micraerophilusGenital anaerobesImmune cellsInflammationPeptostreptococcus anaerobiusPrevotella bivia

Identifiers

PMID41162450
PMCPMC12572226

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.