Evidence map›Paper›PMID 41160376›Full record

ArticleCell biochemistry and biophysics2026

Comparative Phosphoproteomic Profiling and Network-based Interactome Analysis of GRK5 and GRK6.

Jaytha Thomas, Fathimathul Lubaba, Suhail Subair, Althaf Mahin, Athira Perunelly Gopalakrishnan, Prathik Basthikoppa Shivamurthy, Athira C Rajeev, Rajesh Raju

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Cell biochemistry and biophysics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jaytha ThomasThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Fathimathul LubabaThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Suhail SubairThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Althaf MahinThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Athira Perunelly GopalakrishnanThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Prathik Basthikoppa ShivamurthyThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Athira C RajeevThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India.
Rajesh RajuThe Centre for Integrative Omics Data Science is located at Yenepoya (Deemed to be University), Mangalore, Karnataka, 575018, India. rajrrnbt@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

G protein-coupled receptor kinases GRK5 and GRK6 are pivotal regulators of cellular signaling, extending beyond GPCR desensitization to modulate non-receptor pathways critical for neuronal development, immune responses, and oncogenic processes. Leveraging a comprehensive analysis of 3825 human phosphoproteomic datasets, this study identifies predominant phosphorylation sites (pS484 and pT485 in GRK5, pS484 in GRK6) and elucidates their co-phosphoregulated proteins, substrates, and interaction networks. Shared co-phosphoregulated proteins, including BRD9 and RBBP6, underscore conserved roles in mRNA processing and cellular proliferation, essential for maintaining tissue homeostasis and implicated in cancer progression. Divergent functions potentially emerge from sequence distinctions like nuclear localization signal in GRK5 driving neuronal-specific regulation via substrates like MAP1B (cytoskeletal remodeling) and ATRX (chromatin dynamics), while GRK6 palmitoylation anchors immune modulation through targets such as GIT1 (cell migration) and HSP90AA1 (inflammation). Proteogenomic profiling reveals upregulation of both kinases, particularly at pS484, in breast cancer, positioning them as oncogenic drivers in tumor growth and metastasis. These findings illuminate how GRK5 and GRK6 orchestrate balanced yet specialized signaling networks, offering biologically grounded insights for targeted therapies in neurological disorders, autoimmune conditions, and aggressive cancers like triple-negative breast cancer.

Indexed as

G-Protein-Coupled Receptor Kinase 5G-Protein-Coupled Receptor KinasesPhosphoproteinsProteomicsHumansPhosphorylationProtein Interaction MapsSignal TransductionG-Protein-Coupled Receptor Kinase 5G-protein-coupled receptor kinase 6G-Protein-Coupled Receptor KinasesGRK5 protein, humanPhosphoproteinsGPCR signalling co-phosphoregulationGRK5GRK6Immune responseNeuronal pathwaysPhosphoproteomics

Identifiers

PMID41160376

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.