ArticleMedical oncology (Northwood, London, England)2025
Ex vivo evaluation of Allium sativum extract on acute myeloid leukemia cells and leukemia stem cell populations.
Article in Medical oncology (Northwood, London, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- In vitro and in silico evaluation of the synergistic effect of Cytarabine and Zataria multiflora Boiss extract on pre-B acute lymphoblastic leukemia cells (NALM-6 cell line).Medical oncology (Northwood, London, England) · 2026Article
- Harnessing nature's arsenal: next steps for garlic-based therapies in acute myeloid leukemia.Medical oncology (Northwood, London, England) · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite therapeutic advances, acute myeloid leukemia (AML) remains associated with high relapse rates and poor outcomes, especially in elderly or unfit patients. Allium sativum is widely known for its medicinal properties, but its anti-leukemic effects have not been fully explored. This study investigated the cytotoxic and pro-apoptotic potential of an ethyl acetate extract of Allium sativum (EAEAS) using ex vivo mononuclear cells from thirteen AML patients. Cytotoxicity was assessed via MTT assay, while mechanisms of cell death were analyzed using annexin V/propidium iodide staining, DNA fragmentation, mitochondrial depolarization, and caspase 3/7 activation. EAEAS induced a dose-dependent decrease in cell viability, with variable IC₅₀ values across samples. Apoptosis was confirmed through mitochondrial dysfunction, sub-G1 accumulation, and caspase activation. Additionally, we evaluated the effect of EAEAS on two LSC subpopulations using multicolor flow cytometry. Although no statistically significant changes were observed, some patients showed a decrease in LSC frequencies after treatment, suggesting possible selective activity. These findings indicate that EAEAS triggers intrinsic apoptosis in AML blasts and may exert partial activity on stem-like compartments, warranting further evaluation as an adjuvant therapy in AML.
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Registered trials
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